腹膜纤维化是降低腹膜透析效率的主要原因。腹膜透析有效进行取决于滤过膜结构完整，间皮细胞是组成滤过膜重要成分。间皮细胞表型转分化（EMT）是腹膜纤维化起始和可逆环节。TGF-β/Smads通路的激活与间皮细胞EMT密切相关。Smurf2通过选择性对 Smads蛋白调节，其已成为调节TGF-β/Smads通路的新靶点。前期研究表明：具有"健脾益肾，化瘀降浊"功效的扶肾方能够延缓间皮细胞EMT。提出假说：扶肾方基于Smurf2双向调节Smads含量，阻止受体复合物形成；同时维持正性和负性受体Smads蛋白生理平衡，阻断TGF-β/Smads信号通路，防治间皮细胞EMT，阻抑腹膜纤维化。在体和离体实验相结合，探讨扶肾方基于Smurf2调控TGF-β/Smads信号通路防治间皮细胞EMT的机制。课题对于阐明中医药防治腹膜间皮细胞EMT机理，延缓腹膜纤维化，提高腹透长期疗效具有重要意义。

Peritoneal fibrosis is the main cause of decreasing peritoneal dialysis efficiency.Peritoneal dialysis effectively depends on the filtration membrane structure integrity, mesothelial cells is an important component of filtration membrane.Mesothelial cell phenotype transdifferentiation of peritoneal fibrosis (EMT) is the initiation and reversible process.TGF- β/Smads pathway is associated with the mesothelial cell EMT.To the electivity for smads protein, Smurf2 become a new target for regulation of TGF-β /Smads pathway, Previous studies have shown that: with " spleen and kidney, expelling turbid " effect of invigorate the kidney can delay the mesothelial cell EMT.Putting forward the hypothesis:based of Smurf2,Fushen decoction bidirectional regulate smads content, blocking the receptor complex formation;at the same time,maintaining a positive and negative receptor Smads protein physiological balance, inhibiting of TGF-β /Smads signaling pathway, mesothelial cells EMT and peritoneal fibrosis.In vivo and in vitro experiments,to invigorate the Fushen decoction mechanism of Smurf2 regulation of TGF-β/smads signal pathway and mesothelial cells of EMT.Subject to clarify Chinese medicine in prevention and treatment of peritoneal mesothelial cells and the mechainsm of EMT delay the peritoneal fibrosis, has an important significance to improve the long-term effect of peritoneal dialysis.