脑血管病是危害人类健康的常见病和多发病，其危害在人口老龄化日益加剧的今天尤为严重，中风后的血管调控以及神经功能恢复不但是神经科医生关注的问题，也是关乎卒中患者生存质量、减少残疾而亟待解决的社会问题。近年来的研究表明，fibulin-5作为一种细胞外基质分子，对血管内皮、新生血管、细胞间连接和血管的通透性等具有调节作用，缺血性卒中早期，血管严重损伤，我们拟通过脑缺血后fibulin-5的水平调控卒中早期血管的稳定以及恢复期的神经修复并探讨其可能机制。本研究拟采用大鼠MCAo/再灌注模型，应用腺病毒转染、分子生物学及分子影像等技术研究fibulin-5对血脑屏障的调控作用，和通过调节Fmod/NF-κB信号通路及SIK2/CREB信号途径促进缺血性脑损伤后的神经修复和神经功能恢复，研究fibulin-5对相关信号通路关键分子的调控作用，为缺血性脑损伤的神经保护提供新的治疗靶点和实验依据。

Cerebrovascular disease is one common and frequent diseases harmful to human health. The harm it causes has become particularly serious in an aging population today. The regulation of blood vessels and the neurological recovery after a stroke is not only the issue that neurologist concern with, but also a social problem to solve, for quality of life in patients and reducing the disabled. In recent years, researches have shown that, fibulin-5, as a kind of extracellular matrix molecules, could regulate vascular endothelial cells, new blood vessels, intercellular junction and vascular permeability. In early phase of ischemic stroke, the blood vessels are seriously damaged, therefore via cerebral fibulin-5 level, we plan to adjust the stability of the blood vessels, regulate nerve repairs in recovery phase, and discuss the possible mechanisms. In our study, the rat MCAo/reperfusion model is adopted. Adenovirus transfection, molecular biology and molecular imaging techniques are applied to learn the effects of fibulin-5 on blood brain barrier. By adjusting the Fmod/NF-kappa B and SIK2/CREB signaling pathways, promoting the nerve repairs and neural function recovery, the role of fibulin-5 in these signaling pathways is studied. The results will offer new therapeutic targets and experimental basis for nerve protection in ischemic brain injury