芯片材料的组分结构和化学识别决定代谢质谱芯片检测性能。受制于生物体系高复杂度和目标代谢标志物低丰度，现有质谱芯片针对代谢物检测效率低下，在临床应用中极为受限。贵金属与电介质组合会通过等离激元共振，引起局域场增强提升离子化检测效率；基于此设计的贵金属核壳组装体质谱芯片预期具备更强选择性及功能界面效应，可实现生物样本代谢组分析。本项目面向冠心病代谢组临床检测需求，设计成分杂化、结构复合、表面功能化的新型质谱芯片。主要包含以下内容：（一）系统研制一系列基于贵金属核壳组装体的质谱芯片，阐明组分、结构与检测效率之间构效关系；（二）开发选择性探针结合识别代谢小分子，发展靶向物质富集鉴定方法；（三）应对冠心病早期诊断、进展评估临床挑战，应用质谱芯片关联检测血、尿体系代谢组，筛查相关分子标志物及差异疾病通路。本项目有助构建适于临床代谢组分析的实用新型工具，并为理解冠心病发生进展机制提供新思路。

The component, structure, and chemical affinity of materials decide the performance of mass spectrometry (MS) chips for metabolic studies. Conventional MS chips are limited with low detection efficiency for clinical use, due to the high complexity of biological systems and the low abundance of target molecular biomarkers. The combination and morphology of the noble metal particles and dielectric substrates will enhance the ionization efficiency through local field enhancement by plasmon resonance. The designed plasmonic core-shell hybrid chip is expected to have stronger selective ionization efficiency and functional interface effects, and may realize the direct detection and analysis of the metabolome from complex bio-fluids. To meet the clinical needs of the diagnosis and evaluation in coronary heart disease, this project aims to develop MS chips based on noble metal core-shell hybrids. This project includes the following aspects: (1) the systematic preparation of a series of MS chips based on plasmonic core-shell hybrids, to investigate the relationship among component, structure, and detection efficiency; (2) the development of selective probes for bio-recognition of small metabolites, to achieve the in-situ enrichment and identification of targeted metabolites; and (3) the application of MS chips in coronary heart disease, correlating the detection of blood and urine metabolomes to achieve the early diagnosis and progress assessment in clinics. This study will help to construct a practical molecular tool suitable for clinical metabolome detection, and shed light on the investigation of molecular mechanisms in coronary heart disease.