目前，《中国药典》收载的新疆阿魏和阜康阿魏植物资源已极度稀缺，市场流通的主要为多伞阿魏，资源丰富，是新疆干旱地区特有的民族药资源。近年已有学者关注多伞阿魏的抗肿瘤活性，但其物质基础和作用机制尚未见系统研究报道。在我们前期初步验证多伞阿魏能抑制体外胃癌细胞增殖活性的基础上，本课题首先采用MTT和CCK-8法对多伞阿魏不同部位体外抗胃癌细胞活性进行系统筛选；其次，构建胃癌幼鼠模型，通过检测抑瘤率、病理学等指标考察多伞阿魏体内抗胃癌活性与安全性；Western-blot等检测分离胃癌细胞Caspase、Bax、Bcl-2等蛋白因子，初步探索抗胃癌的分子机制。在此基础上，对活性部位进行分离纯化，IR、MS、NMR进行结构解析，并开展构效关系研究。通过本课题研究，阐明多伞阿魏抗胃癌物质基础和作用机制，为丰富抗肿瘤天然产物与促进新疆地区阿魏资源开发利用，加快民族医药产业发展，带动当地经济增长奠定基础。

At present, plant resources of Ferula sinkiangensis K.M.Shen and Ferula fukangensis K.M.Shen, recorded in “Chinese Pharmacopoeia” were extremely scarce. Herbs of Ferula ferulaeoides (Steud.) Korov., which is widely used as national medicine by Uighur native people, is mainly circulated on Chinese herbal medicine market. It is rich in resources, unique distributed in Xinjiang drought regions, China. In recent years, researching of Ferula ferulaeoides was focused on anti-tumor activity, but research on its material basis and mechanism of action have not been systematically reported by scientists. On the basis of anti- gastric cancer cell activity study on extracts of Ferula ferulaeoides in vitro, the first purpose of this project is to screen the active parts of anti- gastric cancer cell activity from Ferula ferulaeoides, anti-gastric cancer cell activity screening assay in vitro were made on different polar parts of Ferula ferulaeoides by MTT and CCK 8. The second purpose of this project is to explore anti-gastric cancer activity in vivo and its safety from Ferula ferulaeoides, by means of detecting indexes of inhibitory rate and more pathology inspection on building baby mice model of gastric cancer. And also purpose of this project is to preliminary study molecular mechanism of anti-tumor of Ferula ferulaeoides by flow cytometry and Western blot detection, separating of gastric cancer cell apoptosis and Caspase, Bax and Bcl-2 protein factors. On the basis of biological activities study on Ferula ferulaeoides in vitro, several purification and separation methods (column chromatography on silical gel, polyamide, Sephadex LH-20) and many kinds of structure elucidation and identification technologies (UV, IR, NMR) were used, and to carry out the structure-activity relationship study between them. Through this project research, clarifying the material basis and mechanism of action from Ferula ferulaeoides fighting anti- gastric cancer cell activity, and then to rich for the anti-tumor natural products, promoting development and utilization of Ferula resources in Xinjiang region, and further accelerate the development of national pharmaceutical industry, driveing the local economic growth.