治疗大肠杆菌性犊牛腹泻病的蒙兽药及其有效成分的调理肠道菌群、抗炎免疫机理研究

细菌对抗生素产生耐药性和动物性食品抗生素残留，成为影响人畜健康和食品安全的重大难题。为此，用草药等调控机体生理机能、提高抗炎免疫力成为趋势之一。大肠杆菌腹泻是犊牛死亡和发育阻滞的主因。蒙药巴布7临床治愈腹泻达95%以上，具有抑菌抗炎免疫作用。为研究蒙兽药在牛源野生大肠杆菌性腹泻中调理肠道菌群及抗炎免疫作用机理，采用生理、药理、分子生物学方法、ELISA、流式细胞术等，在细胞分子层面研究血液免疫指标在其发病机制中的作用、致腹泻病大肠杆菌对肠黏膜机械和免疫屏障包括Payer's结的影响机理；研究蒙兽药及其有效成分调理肠道菌群、治疗腹泻对机体抗炎免疫机能、肠黏膜机械和免疫屏障的影响机制，并通过抗炎免疫细胞信号转导机制研究，筛选主要激活的上下调基因，从基因和蛋白表达水平探索蒙兽药有效成份的抗炎免疫机理。为防控危害犊牛健康的腹泻病、培育高产健康牛和蒙药替代抗生素提供理论依据。是前期研究基础上的创新。

Bacterial resistance to antibiotics and animal food antibiotic residues, become a major problem that affects the human and animal health and food safety. To this end, use herb medicines regulating the body's physiological function, improve the anti-inflammatory increasing-immune to become one of the trends. E. coli diarrhea is a main reason of causing calf death and growth retardation. Mongolian medicine babua 7 in clinical cure diarrhea is more than 95%, has antibacterial anti-inflammatory increasing-immune function. For the study of Mongolian veterinary medicine treatment diarrhea in regulating-bacterium of intestine anti-inflammatory immune mechanism caused by bovine coming wild E. coli, physiology, pharmacology, molecular biology methods, ELISA and flow cytometry technology be used, to research the role of blood immune index in its pathogenesis, the influence mechanism causing diarrhea E coli in intestinal mucosal mechanical barrier and immune barrier, including the Payer 's knot, in cell and molecular level; to research Mongolian veterinary medicine and its effective components on regulating intestinal bacterial, treatment diarrhea mechanism by influencing of anti-inflammatory and immune function, the intestinal mucosal mechanical and immune barrier mechanism, and to research cell signal transduction mechanism of its anti-inflammatory increasing-immune, screening mainly activated gene, to explore anti-inflammatory immune mechanism of Mongolian veterinary medicine and its effective ingredients on the level of gene and protein expression . To provides the theory basis for prevent and control harmful calf diarrhea disease, high-yielding healthy cows with Mongolian instead of antibiotics. It is the original research on the early basis stage.