人巨细胞病毒（HCMV）与炎症相关性青光眼尤其是青睫综合征的关系越来越引起关注，但其致病机制尚不清楚。我们前期研究发现，青睫综合征患者房水中分离出的HCMV为gB3基因型，而巨细胞病毒性角膜内皮炎为gB1型，提示HCMVgB基因分型在上述疾病的发生中起关键作用。目前未见HCMVgB基因分型与炎症相关性青光眼关系的研究报道。依据前期研究及文献分析，我们提出假说：引起青睫综合征和角膜内皮炎的HCMV具有不同gB基因型，由于gB基因介导的前房细胞感染嗜性不同，导致产生不同的疾病。本研究拟从gB基因型这一新角度出发，将临床分离HCMV体外扩增后建立动物模型，分析不同基因型HCMV对前房内组织细胞的感染嗜性，并观察炎症反应特点及细胞病理改变，探讨HCMVgB基因分型与青睫综合征及角膜内皮炎的关系。研究结果将进一步明确炎症相关性青光眼的病因及发病机制，为临床上这类疾病的诊断和治疗提供新的实验依据。

The relationship between human cytomegalovirus (HCMV) and glaucoma associated with inflammation, especially glaucomatocyclitic crisis, has drawn more and more attention. However, the mechanisms still remain unclear. Our previous study has found that HCMV genotype was gB3 for the viral strain isolated from the aqueous humor of glaucomatocyclitic crisis patients. However, the HCMV genotype was gB1 in corneal endotheliitis patients. The results indicate that HCMV gB genotype may play a key role in the pathogenesis of these diseases. So far, there is no report on the relationship between HCMV gB genotype and glaucoma associated with inflammation. Based on our previous study and published literatures, we hypothesize that: viral tropism to cells in the anterior chamber is determined by HCMV gB genotypes, which results in different clinical manifestations in corneal endotheliitis and glaucomatocyclitic crisis. The purpose of this study is to explore the role and mechanism of HCMV gB genotype in the pathogenesis of glaucoma associated with inflammation. HCMV strains are isolated from aqueous humor of glaucomatocyclitic crisis and corneal endotheliitis patients and amplified in vitro. Animal model is established by anterior chamber injection. Pathological changes and inflammatory reactions in the anterior section are evaluated to explore the viral tropism of different HCMV genotypes and its relationship with corneal endotheliitis and glaucomatocyclitic crisis. The results of this study will further clarify the etiology and pathogenesis of glaucoma associated with inflammation and provide a new experimental basis for the diagnosis and treatment of these diseases.