制川乌-白芍功能祛风除湿，通痹止痛，是"中医外治"法的常用药对。目前对该药对配伍"增效减毒"机理的研究主要集中在"化学成分"和"药理效应"的层面，而给药方式多为口服和注射，故其研究成果并不能推及到外用给药，亦无法阐明"中医外治"组方规律。本项目在中医药理论和前期研究的基础上，提出 "制川乌-白芍配伍经皮给药后，可通过影响活性成分在皮肤局部药动学行为及在组织器官的分布与代谢，达到"增效减毒"的目的"的假说，采用微透析、RT-PCR、Western Blot等技术，综合应用生物药剂学、分子生物学等方法，考察配伍前后皮肤局部药动学参数、P450酶、P-gp的变化及作用规律，从而使该药对配伍机理得到阐明。本项目从经皮转运的角度研究配伍机理，为配伍理论研究提供新思路；以药对为基点阐明"中医外治"组方规律，为外用制剂配伍研究提供示范；在中医药理论的指导下寻找促进剂，为吸收促进剂的开发提供新视角。

The couplet medicines processed Radix Aconiti-Radix Paeoniae Alba is used to treat rheumatism Arthralgia, and it is also widely used in topical formulations. Currently, compatibility mechanism studies of these couplet medicines to investigate its changes on chemical constituents and pharmacological effects are often administered by oral or by injection. And there is no report to clarify its mechanism of the "enhancing efficiency and reducing toxicity" based on the percutaneous route and intimate process. This project provide a hypothesis that" The couplet medicines processed Radix Aconiti-Radix Paeoniae Alba delivered by transdermal can reach the purpose of enhancing efficiency and reducing toxicity as it can affect the dermatological local pharmacokinetic behavior and the distribution in the tissues and organs" based on chinese medicine theory and previous experiments. Microdialysis、Western Blot and other technologies integrated with bio-pharmacy and molecular biology research methods are used to investigate the local pharmacokinetic parameters of skin before and after compatibility, the cytochrome P450 enzyme, the change and interaction law of P-GP. This project is to clarify the "enhancing efficiency and reducing toxicity" compatibility mechanism of the couplet medicines processed Radix Aconiti-Radix Paeoniae Alba based on the percutaneous route. It can provide theoretical support for the clinic use of these couplet medicines, provide a theoretical basis for the prescription compatibility of topical preparations, and it also can provide new perspectives and approaches for the research of traditional Chinese medicine.