长非编码RNA 在肝癌发生发展中的作用及机制

肝癌是我国常见恶性肿瘤之一。近年来的研究揭示长非编码RNA（lncRNA）在肿瘤发生发展中具有扮演了重要角色，然而相关研究仍处于起步阶段。我们前期发现lncRNA-URH（lncRNA Up-Regulated in HCC）在肝癌组织中表达显著上调，且抑制其表达显著抑制细胞增殖，并可促进细胞凋亡，还可促进细胞迁移与侵袭的能力，提示lncRNA-URH可能参与调控肝癌的发生发展。在此基础上，本项目拟结合体内外模型开展以下研究：1.分析lncRNA-URH对肝癌细胞增殖、凋亡及转移等恶性表型的调控作用及对肝癌发生发展的影响；2.鉴定与lncRNA-URH相互作用的RNA和蛋白质，揭示lncRNA-URH调控肝癌发生发展的分子机制；3. 揭示lncRNA-URH在肝癌中表达异常的分子机制。所得结果将有助于阐明lncRNA-URH的功能和调控网络，并可为制定有效的抗肿瘤治疗策略提供理论基础。

Hepatocellular carcinoma is one of malignancies in China. Recent studies suggested that dysregulation of long non-coding RNAs plays an important role in the development and progression of tumors. Our previous studies have showed that a long non-coding RNA (named lncRNA-URH), were aberrantly over-expressed in HCCs. In addition, knockdown the expression of lncRNA-URH significantly suppressed proliferation, and promoted apoptosis, along with the decreased migration and invasion of the HCC cell lines, which suggest a role of lncRNA-URH the development and progression of HCCs. With these findings, we planned to use in vitro and in vivo models to study the function and its underlying mechanisms of lncRNA-URH: 1) effects of the regulatory roles of lncRNA-URH in the malignant characteristics of tumor cells and the development and progression of HCCs; 2) the underlying mechanism in lncRNA-URH mediated development and progression of HCCs; 3) the molecular mechanisms underlying the deregulated expression of lncRNA-URH. The results obtained will not only reveal the biological function and regulatory networks of lncRNA-URH, but also provide theoretical basis for the clinical antitumor therapy of HCCs.