拟南芥中介体蛋白MED25参与JAZ基因可变剪切调控的分子机制研究

JAZ是茉莉酸信号转导途径中最重要的组分之一，JAZ的表达受可变剪切调控。然而，参与调控JAZ可变剪切的组分仍不清楚。我们已有的研究表明MED25可通过与MYC2相互作用在转录水平上调控JAZ的表达，进一步的研究表明，MED25还可参与JAZ4的可变剪切调控，此外，MED25与剪切因子PRP39存在着直接的蛋白相互作用，表明MED25可能通过与剪切因子的相互作用参与JAZ4的可变剪切调控，继而介导JA信号通路的调节。然而，PRP39是否直接参与JAZ4的可变剪切调节及MED25是否通过PRP39影响JAZ4的可变剪切进而影响JA信号通路仍不清楚。本项目拟采用遗传学、分子生物学及生物化学手段探讨MED25是否通过PRP39介导JAZ4的可变剪切调节继而参与JA信号通路的调控。本研究将揭示MED25在转录后水平参与JA信号转导调控过程的分子机制，丰富JA信号转导调控网络。

JAZs are one of the most important components in JA signal transduction pathway, and the expression of JAZs are regulated by alternative splicing. However, little is known about the components in regulating the alternative splicing of JAZ.Our previous results indicated that MED25 could regulate JAZs expression under transcriptional level by interacting with MYC2, our further researches also showed that MED25 could mediate the alternative splicing of JAZs. In addition, MED25 could interact with plant splicing factor PRP39 directly, which means that MED25 might regulate alternative splicing of JAZ through interact with splicing factor. However, the exactly molecular mechanism of whether splicing factors are involved in the regulation of the JA signal transduction and the close linking between MED25 mediates JA signal tranduction and alternative splicing of JAZs remains unknown. Our project is plan to combine genetics, molecular biology and biochemistry approaches to discuss whether these mediators could regulate JA signal transduction through alternative splicing of JAZs, furthermore, we want to analyse whether these mediator-interacted splicing factors are also involved in JA signal transduction. Our research would help us to clarify molecular mechanism of the MED25 during JA signal transduction in post-transcription level.