近年来，新城疫病毒跨种感染宿主的范围不断扩大，本实验室分离到猪源新城疫病毒JL01株，通过动物回归试验证实该病毒对猪的致死性感染。并通过前期研究确定了该病毒的感染和致病性与HN蛋白跨膜区（119-120位氨基酸）相关，但新城疫病毒跨种感染的机制仍不清楚。V蛋白在新城疫病毒的致病性和宿主嗜性等方面具有重要作用，因此本项目拟采用反向遗传操作技术，以猪源新城疫病毒JL01株为模板构建感染性克隆，分别拯救出V蛋白编码基因缺失株、新城疫病毒弱毒株和强毒株V蛋白基因替换株等系列重组新城疫病毒。对拯救的重组新城疫病毒进行生物学特性鉴定的基础上，用纯化的重组病毒分别感染猪呼吸道细胞系（肺上皮细胞和呼吸道上皮细胞），利用差异蛋白组学方法研究重组病毒感染后的宿主细胞蛋白变化，分析V蛋白变异的重组新城疫病毒对猪呼吸道细胞感染性和致病性变化，确定V蛋白在猪源新城疫病毒跨种感染中的作用机制。

Recently, cross-species infetion of the host range of Newcastle disease virus continues expanding. The swine original Newcastle disease virus JL01 had been isolated by our lab, and the regression test showed that the virus could .induce piglet death. And our former studies showed that HN protein(119 and 120 aa mutation) was related to the infection ability of swine Newcastle disease virus JL01. But until now the mechanism of Newcastle disease virus on cross-species infection is not known, the V protein of Newcastle disease virus is related to the pathogenicity and host tropism. The reverse genetics will be used to construct the infectious clone of Newcastle disease virus JL01, and then several recombinant Newcastle disease virus will be rescued such as V protein deletion strains, V protein avirulent replacement strains and virulent replacement strains. On the basis of the biological characterization of recombinant Newcastle disease virus rescued, the porcine respiratory cells (lung epithelial cells and airway epithelial cells) were infected with purified recombinant virus. The host cell protein changes were detected by differenial proteomic method, and the infection and pathogenicity of porcine respiratory tract cells change of the recombinant virus V protein deletion and replace strains will be analysised. Finally, the mechanism of V protein of swine Newcastle disease virus on cross-species infection will be disclosed.