从ERK、p38信号通路探讨枢经推拿对大鼠慢性神经病理性疼痛的影响及镇痛机制研究

慢性疼痛是一种长期以来威胁着人类健康的病症，由于其病理机制的复杂性，目前缺乏有效及特异性的干预治疗手段。细胞内信号转导途径在研究慢性神经病理性疼痛方面受到越来越多的关注。研究表明ERK、p38信号通路对神经病理性疼痛的发生发展起着重要的作用。. 前期课题研究显示枢经推拿对缓解疼痛效果明确，但其潜在机制尚未清楚，因此本项目在此基础上，基于ERK、p38信号通路，应用Western blot和Real-time PCR等技术，观察枢经推拿对慢性神经病理性疼痛大鼠脊髓膨大段信号分子Raf、ERK 、CREB、 MKP-1、p38的蛋白及基因表达的影响，从细胞分子水平揭示枢经推拿镇痛的潜在作用机制，为枢经推拿干预慢性神经病理性疼痛提供科学依据。

Chronic pain is a disease that has been threatening human health for a long time. Due to the complexity of its pathological mechanism, it still lacks of effective and specific interventions. Recent years, intracellular signal transduction pathways have gained more and more attention in the study of chronic neuropathic pain. Certain study shows that the ERK, p38 signaling pathway is playing an important role in the development of neuropathic pain.. Former study has showed that there is an definite pain relief effect by Shu Meridian Tuina however its latent mechanism remains unclear. Based on the foundation of this and ERK, p38 signaling pathway, to study the effect of Shu Meridian Tuina on protein and gene expression of signal molecules Raf, ERK, CREB, MKP-1、p38 in the spinal cord swelling period of rats with chronic neuropathic pain using Western blot and Real-time PCR technique ，finally to reveal the potential analgesic mechanism of Shu Meridian Tuina on cellular and molecular level and provide scientific evidence for intervention of Shu Meridian Tuina on chronic neuropathic pain.