本项目以百草枯敏感型牛筋草为外植体，优化牛筋草组培再生体系培养条件，筛选农杆菌侵染牛筋草愈伤组织的条件，建立利用农杆菌介导获得转基因牛筋草植株的遗传转化体系，为牛筋草抗药性基因等遗传特性的研究打下良好基础；以百草枯抗性型牛筋草为模板，克隆百草枯抗性相关的多胺及转运体基因，通过构建正反义表达载体，遗传转化获得转基因牛筋草，鉴定该基因对百草枯的抗性作用。本项目对阐明百草枯抗性机理有重要意义，为科学施药、延长百草枯的使用寿命提供参考依据，同时为除草剂抗药性转基因研究提供理论基础。

This project will establish goosegrass (Eleusine indica L.) genetic transformation system mediated by Agrobacterium tumefaciens, with optimizing the culture condition of the goosegrass tissue culture and regeneration system with paraquat-susceptible goosegrass used as explants, and selecting the condition of Agrobacterium infected goosegrass callus. It will be a good base to research the hereditary character of goosegrass such as herbicide resistant. The polyamines and transporter genes will be cloned using paraquat-resistant goosegrass as template. Through constructing over and antisense expreseeion vector, the genes will be transferred into goosegrass by Agrobacterium to process its functional verification finally. The project will have important significance to elucidate the resistance mechanism of paraquat, provide reference to scientific paraquat application, and promoting the study of herbicide transgenic organism.