经济作物甜高粱耐盐碱分子机制及种质创新

黄河三角洲具有以东营等地为主的大量盐碱地，其特点是土地形成时间短、盐碱含量高及土壤有机质含量低，这样的土地一般不适合普通农作物生长。黄河三角洲农高区根据实际情况布局发展的一个重要方向是种植耐盐碱经济作物饲草，发展以草食动物为主的畜牧业。甜高粱是耐贫瘠、耐盐碱的优质高产且宜于反刍动物食用的饲料经济作物，并因其秸秆含糖量高还可用做生物能源原料。本项目拟基于当地具体需求，结合实验室与东营盐碱地的实际情况，联合基础研究优势单位，以全基因组关联分析和极端盐碱反应的自交系的RNA-seq分析数据为基础，结合杂交后代的QTL分析，利用遗传学、细胞生物学和分子生物学等手段分离高粱耐盐碱相关SNP模块及基因，研究其耐盐碱的分子机制，为甜高粱等经济作物以及其它农作物的耐盐碱育种提供理论基础；并将与盐碱相关联的SNP开发为分子标记，用于耐盐碱高产优质甜高粱的育种中，培育适合黄三角种植的耐盐碱高粱和甜高粱新种质。

The Huanghe (Yellow River) Delta, especially the area around Dongying, is the new formed land with high saline-alkali and low organic matter content. The land is usually not suitable for the general crop growth. By analyzing the current situation and local demands for agriculture, the local government published the long term plan encouraging to plant saline-alkali tolerant grasses or economic crops to develop the livestock farming. Sweet sorghum, known as the high saline-alkali economic crop with high biomass and sugar content in stem, and high protein content in leaves, can be used as high quality forage or biofuel stock. This research project will base on the local demands to form collaboration team from Institute of Genetics and Developmental Biology-CAS and Shandong Normal University. The team will adopt the local saline-alkali land to plant large scale of sorghum population to preform GWAS, RNA-Seq and QTL analysis. Together with the genetics, cellular biology and molecular biology techniques, we will isolate those saline-alkali tolerant SNPs and genes to study the mechanism for saline-alkali tolerance in sorghum. Based on the investigating results, we will design related techniques, such as molecular markers to breed new varieties of sorghum and sweet sorghum with the high biomass and good quality, which are suitable for planting in the Huanghe Delta land.