基于ICG-PD-L1探针的近红外二区荧光成像技术用于识别泡型包虫病肝内微小病灶的研究

泡型包虫病（Alveolar Echinococcosis，AE）是严重危害人民健康的一种人畜共患寄生虫病，传统影像学检查难以早期识别肝AE微小病灶，导致漏诊、误诊或治疗不规范。近红外二区（NIR-II）荧光成像技术具有高精准度和空间分辨率，是生物医学光学成像领域的研究热点，而其在AE诊治方面的研究尚未报道。本项目拟通过：搭建并优化NIR-II荧光宏观/显微成像系统；建立不同阶段AE动物模型；以吲哚菁绿（ICG）为NIR-II荧光探针实现AE病灶的负显影；制备靶向于AE并且具有NIR-II发光特性的ICG-PD-L1复合物并探究其制备机制；最后以ICG-PD-L1为NIR-II荧光/靶向多功能探针，实现肝AE微小病灶的特异性成像。本项目旨在探究ICG-PD-L1辅助的NIR-II荧光成像技术在精准识别肝AE微小病灶的可行性和有效性，为AE的早期诊断提供新的方法和思路。

As a kind of parasitic zoonosis, alveolar echinococcosis (AE) seriously endangers people's health. It is difficult to identify the small lesions of AE in the early stage by traditional imaging examination, which leads to missed or misdiagnosed. Near-infrared II (NIR-II) fluorescence imaging technique has has become a research hotspot in bio-imaging areas thanks to the high accuracy and spatial resolution, however, the study of NIR-II fluorescence imaging in AE theranostics has not been reported yet. This project plans to: Build and optimize the NIR-II fluorescence macro-/micro-imaging system; Establish AE animal models in different stages; Utilize the indocyanine green (ICG) as the NIR-II fluorescence probe to achieve the hypoechoic enhancement of AE lesions; Prepare the AE targeting ICG-PD-L1 complex with NIR-II fluorescence characteristics and further explore the preparation mechanism; Finally, utilize the ICG-PD-L1 complex as the NIR-II fluorescence/targeting multi-functional probe to achieve the specific imaging of the AE micro-lesions. This project aims to explore the feasibility of ICG-PD-L1 assisted NIR-II fluorescence imaging technology in accurate identification of AE micro-lesions, and provide new methods and ideas for early diagnosis of AE.