辣椒果实Vc合成关键基因pvtc的克隆与功能解析

我国的辣椒年栽培面积达3000万亩，在蔬菜生产中占据重要地位。同时辣椒是公认的果实Vc含量最高的蔬菜，但不同品种之间果实Vc含量差异非常显著，果实高Vc含量品种的选育已成为辣椒品质育种的重要方向。目前辣椒果实Vc含量相关的分子生物学研究进展缓慢，至今未见QTL精细定位和基因克隆的报道，阻碍了高效准确分子育种技术的研发和果实高Vc含量品种的选育进程。克隆调控果实Vc生物合成的关键基因并解析其分子机理，对于辣椒品质分子育种具有重要意义。项目组前期通过QTL定位和转录组测序，率先筛选到了调控辣椒果实Vc合成的关键基因pvtc。本项目拟以此为基础，克隆pvtc基因，利用已经建立的辣椒遗传转化体系进行功能验证，解析基因调控表达的分子基础。研究结果将进一步丰富植物Vc代谢网络的理论知识，为辣椒果实高Vc含量分子育种提供技术支撑，加速果实高Vc含量辣椒新品种的选育进程。

Pepper plays an important role in Chinese vegetable production, with the annual cultivated area in China of 30 million mu. At the same time, pepper is recognized as the vegetable with the highest Vc content in fruit, but there are significant differences in Vc content among different varieties. Breeding varieties with high Vc content in fruit has become an important direction of pepper quality breeding. The progress of molecular biology of Vc content in pepper is still limited, and so far there is few reports of QTL mapping or gene cloning, which hinders the development of efficient and accurate molecular breeding of pepper varieties with high Vc content in fruit. It is of great significance to clone and analyze the key gene of Vc biosynthesis in pepper molecular breeding for quality. At the earlier stage, using QTL mapping and transcriptome sequencing, the project group first screened the key candidate gene pvtc for pepper regulating Vc synthesis , which has laid a good foundation for cloning the target gene and analyzing the molecular mechanism of Vc synthesis in pepper fruit. Based on this, the project plans to clone pvtc gene, perform gene function verification using the established genetic transformation system of pepper, and analyze the molecular basis of gene regulation and expression. The research results will further enrich the theoretical knowledge of plant Vc metabolism network, provide technical support for the molecular breeding of pepper with high Vc content in fruit, and accelerate the breeding process of new pepper varieties with high Vc content in fruit.