可见/近红外光激活转移荧光染料的构建及其应用

单分子定位超分辨成像技术的发展为生命科学研究提供了突破光学衍射极限的研究工具。光激活定位等显微技术实现超分辨荧光成像的核心要素之一是高性能、光可调的荧光染料。然而，目前能用于单分子定位超分辨成像的光可调的小分子荧光探针，尤其是光转移小分子荧光染料（激发光谱和发射光谱可以通过光照来移动改变）的种类非常有限。本项目旨在1）发展一类新颖的亚砜连接氟硼二吡咯类可见/近红外光激活转移有机小分子荧光染料，研究其通过可见/近红外光激活释放SO小分子生成大共轭体系的氟硼二吡咯二聚体染料，实现吸收和发射的超过100 nm光谱红移的光激活性能；2）研究光去笼反应机理，通过取代基调控染料分子稳定性和光转移反应活性；3）发展可定位的特异性光转移染料，研究其在单分子定位超高分辨成像中的应用。项目发展的新型光笼染料将为进一步构建光转移小分子染料提供理论支撑，推动小分子染料在单分子定位超分辨成像中的进一步应用。

The development of single molecule localization microscopy based super-resolution imaging technology provides a tool to break through the optical diffraction limit for life science research. One of the key elements of super-resolution fluorescence imaging such as photoactivation localization fluorescence microscopy is a high-performance fluorescence dye with the ability to control the on−off state of fluorescence. However, currently, there are only a limited number of switchable small molecule fluorescent probes that can be used for single-molecule localization super-resolution imaging, especially for photoconvertable small molecule fluorescent dyes (excitation spectrum and emission spectrum can be moved and changed by light). . In this project, we will develop develop a series of novel sulfoxide connected BODIPY dyes as visible/near-infrared light activated photoconvertable small organic fluorescent dyes, and study the formation of BODIPY dimers under visible/near-infrared light by releasing SO molecules, achieving more than 100 nm red-shifts of absorption and emission maxima; We will also investigate the photodecaging mechanism reaction, and optimize the stability and photoconvertable reactivity through varing substituents; Based on the structure-properties relationship investigation, we will further develop several novel photoconvertable dyes with targeting groups and study their application in single molecule localization microscopy. . The photodecaging dyes developed in this project will provide theoretical support for further design of photoconvertable organic small molecule dyes and will also promote the application of organic small molecule dyes in single molecule localization microscopy.