乙烯和生长素的生物合成和信号转导途径已较为明晰，研究两种激素的关系将加深对植物激素调控网络的认识。半水生单子叶水稻的乙烯反应有其保守性和特殊性。本研究中，我们筛选到一个根特异的乙烯不敏感水稻突变体mhz10，克隆了突变基因MHZ10,验证了其在根部乙烯反应中的功能。本项目将着重解析MHZ10的转录调控机制。前期研究表明乙烯信号转导途径OsEIL1直接转录激活MHZ10，促进根部生长素合成。另外，我们还获得了生长素途径通过Aux/IAA作用于OsEIL1，正反馈调节MHZ10表达，进而促进乙烯反应中生长素合成的初步证据。本项目后续将重点研究OsEIL1和Aux/IAA蛋白互作、转录调控活性及活性差异决定因素、相关信号转导组分协同整合等几方面内容，解析OsEIL1和Aux/IAA如何共同调控MHZ10及其参与的根部乙烯反应，并有望揭示乙烯和生长素途径在信号转导水平上互作的新机制。

At present, the biosynthesis and signaling pathways of ethylene and auxin are mostly clear. Further study of the interplay between them will help us fully understand the plant hormone regulatory network. Monocotyledon rice has a “semi-aquatic” habit, and its ethylene response are both conservative and specific. In this project, we screened a root-specific ethylene-insensitive rice mutant mhz10. We first cloned the corresponding gene MHZ10, and verified its function in the root ethylene response. We will focus on the detailed mechanisms of the transcriptional regulation of MHZ10 in the following study. Through previous studies, we have confirmed that OsEIL1 directly activates MHZ10 transcription, and promotes auxin biosynthesis in rice roots. In addition, we caught some preliminary evidences that the Aux/IAA in auxin pathway may interacts with OsEIL1, and there may be a positive feedback acting on the ethylene-upregulated MHZ10 transcription, thereby promoting auxin biosynthesis in the ethylene response. Several aspects of physical interaction between OsEIL1 and Aux/IAA, transcription regulation activity of Aux/IAA to OsEIL1, the determinants of the different activities, and the integration of the related signaling components will be included in the continuous studies, and it will hopefully reveal new mechanisms for the interaction of ethylene and auxin pathways at the level of signaling.