静磁场作用下LncRNA作为ceRNA介导miR-466d靶向抑制MMP13促进成骨分化的作用机制研究

骨代谢与口腔疾病临床治疗密切相关，静磁场作为一种安全的物理因素对骨代谢有明显的调控作用。大量研究表明microRNA在调控骨代谢进程中发挥了关键作用，LncRNA也被证实可以作为ceRNA吸附microRNA参与调节骨代谢。本课题组前期研究发现静磁场作用下miR-466d能靶向抑制MMP13从而促进成骨，且研究结果提示lnc_LOC101928134可能通过靶向调控miR-466d进而影响成骨细胞的成骨分化。本项目拟采用RNA干扰技术探讨lnc_LOC101928134对骨代谢的影响，运用荧光素酶报告载体、慢病毒高/低表达、RNA结合蛋白免疫沉淀等技术探讨lnc_LOC101928134作为ceRNA结合miR-466d影响骨代谢的分子机制，阐明静磁场调控骨代谢的分子生物学机理，丰富磁生物学理论基础，并寻找骨改建过程中的关键靶点，为骨代谢相关口腔疾病靶向治疗探索新思路。

Bone metabolism is closely related to the clinical treatment of oral diseases. As a safe physical factor, static magnetic field can obviously regulate bone metabolism. A number of studies have shown that microRNAs play a key role in regulating bone metabolism, and long non-coding RNAs have been shown to act as a microRNA adsorber on ceRNA to regulate bone metabolism. Our previous research showed that miR-466d could inhibit MMP13 and promote osteogenesis, and the results suggested that lnc_LOC101928134 might affect osteoblast differentiation by targeting miR-466d. The aim of this study was to investigate the effect of lnc_LOC101928134 on bone metabolism by RNA interference, using luciferase reporter vector, lentivirus high/low expression, RNA binding protein immunoprecipitation and other techniques to explore the molecular mechanism of bone metabolism affected by lnc_LOC101928134 as ceRNA binding miR-466d, and to elucidate the molecular biological mechanism of static magnetic field regulating bone metabolism, to enrich the theoretical basis of magnetic biology, and to find the key targets in the process of bone remodeling, and to explore new ideas for targeted treatment of bone metabolism-related oral diseases.