MyoD 基因家族包括MyoD1、MyoG、Myf5和Myf6，对肌肉的产生和动物生长具有重要意义，而MyoD1在骨骼肌特异性基因的激活与转录过程中起着总开关的作用，并参与骨骼肌生长、发育和修复。本课题组前期研究了MyoD1在牛各组织中的表达谱，筛选和鉴定了MyoD1启动子区及其相关转录因子，并从细胞和分子水平探讨了MyoD1启动子的组织特异性。在前期研究工作基础上，本课题拟进一步研究在牛成肌细胞中，MyoD1启动子过表达前后细胞中的差异基因，筛选牛MyoD1启动子调控的目的基因；研究筛选的目的基因在牛成肌细胞及过表达细胞中的表达差异，验证牛MyoD1-目的基因转入小鼠后对其生长的影响，弄清MyoD1启动子调控目的基因的有效性和特异性，解析牛MyoD1启动子调控目的基因靶向转录的分子机制。

MyoD gene families that include MyoD1, MyoG, Myf5 and Myf6 have important significance for the generation of muscle and animal growth. While MyoD1 plays the role of master switch in the process of transcription and activation of the specific genes, and participates in the growth, development and repair of skeletal muscle. Previously, our research group studied the expression profile of MyoD1 gene in cattle tissues, screened and identified the start activity of MyoD1 promoter and the related transcription factors, and also explored the tissue specificity of MyoD1 promoter in terms of cellular and molecular levels. On the basis of previous work, the differential genes caused by the deficiency and over-expression of MyoD1 promoter were identified in bovine myoblasts to screen the MyoD1 regulated genes. Moreover, the expression difference of MyoD1 regulated genes in the normal and over-expressed bovine myoblasts was also investigated. Furthermore, the growth of mice transferred by the MyoD1 regulated genes was observed to clarify the effectiveness and specificity of MyoD1 promoter. These obtained results will promote the understanding of the molecular mechanism of gene targeting transcription regulated by MyoD1 promoter.