疼痛包括痛感受和痛情绪，虽然痛情绪严重危害患者的身心健康，但对它的研究却远滞后于对痛感受的研究。本研究的目的是：用后脚掌注射CFA结合条件性位置逃避的行为测试，使用药理学、免疫组化、Western-blot和在体多通道电生理技术，揭示电针刺激环跳穴（GB30）缓解痛情绪的机制。研究表明，前扣带回皮层（ACC）NMDA受体参与疼痛情绪反应的形成。我们前期的研究提示电针可激活吻端ACC（rACC）阿片系统缓解痛情绪。预实验发现：电针可明显地抑制rACC内NMDA受体亚单位NR1的磷酸化；NMDA受体和μ受体共表达于rACC神经元。据此，我们假设：电针刺激引起rACC释放的内源性阿片肽，可激活阿片受体，通过抑制NMDA受体亚单位NR1，NR2A和NR2B的磷酸化，调节rACC神经元的放电活动，从而缓解痛情绪。本研究将揭示痛情绪的发生以及电针缓解痛情绪的机制，为抗痛治疗提供新的理论基础和新的思路。

Pain is a disease, and also the common symptoms of various illnesses, that has been being particularly difficulty in cure, and brought enormous economic and emotional burden in society. Pain-related affective response, an important component of pain, heavily reduces patients' quality of life, but research of affective pain is largely lagged behind that of sensory-discriminative component of pain. The mechanisms of the affective pain and the therapeutical targets are not determined. Researches show that anterior cingulate cortex (ACC) play key role in pain-related affective response. Our previous study demonstrated that electroacupuncture (EA) significantly alleviated affective pain through activating rostral (rACC) opiate system in a model of inflammatory pain. It has been found by pilot experiments that EA markedly inhibited the phosphorylation of NR1 in rACC; the NMDA receptors are co-expressed with μ receptors in rACC neurons. We hypothesis that EA can increase the release of endogenous opioid, and activate opioid receptors, which then inhibit the phosphorylation of NR1, NR2A and NR2B in rACC, thereby alleviate pain-related affective response. In this study, we will apply CFA-injected inflammatory pain model in hind paw and conditioned place avoidance (CPA) test, and use multichannel data acquisition system, Double immunofluorescence and Western-blot technologies, to explore the mechanisms that EA stimulate Huantiao (GB30) acupoint to alleviate pain-related affective response, and provide new ideas for exploring the mechanisms on affective pain and treat chronic pain.