Nurr1基因在神经干细胞（NSCs）与小胶质细胞联合移植治疗帕金森病中的作用

帕金森病（PD）是第二大神经退行性病变。传统的多巴胺替代疗法，只能改善症状，并不能阻止病情发展。干细胞移植治疗是近年来被认为极有希望的治疗手段之一。然而，单纯干细胞移植治疗，未能改变黑质纹状体的"微环境（niche）"，移植的干细胞在局部难以存活。因此，本课题通过体外和体内两种方案研究微环境关键细胞- - -小胶质细胞与神经干细胞相互作用。在小胶质细胞中过表达Nurr1，既抑制其释放炎症因子，又能促进其分泌神经营养因子；神经干细胞（NSCs）过表达Nurr1，能促进其向多巴胺能神经元分化。二者联合移植，修复受损的多巴胺能神经元，改善PD症状。

Parkinson's disease (PD) is the second most common degenerative disorder of the central nervous system (CNS). Traditional dopamine replacement therapy may alleviate the symptoms of PD, while which could not stop the development of PD. Stem cell transplantation is considered as one of the most hopeful therapeutic methods for treatment of PD. However, transplantation of stem cells can't change the microenviroment (niche) in the substantia nigra and striatum, with little transplanted cells surviving. Therefore, we will explore the interaction between neural stem cells (NSCs) and microglia cells (the key cell of niche) through in-vitro and in-vivo research. Over expression of Nurr-1 gene in microglia cells, can suppress the releasing of inflammatory factors and boost the releasing of neurotrophic factors from microglia cells. Over expression of Nurr-1 gene in NSCs, can promote the dopaminergic differentiation of NSCs. Co-transplantation of Nurr-1 gene modified microglia cells and Nurr-1 gene modified NSCs can improve the local niche, with more transplanted cells survive and replace the impaired dopamine neurons, and cure PD finally.