蔗糖转化酶与蔗糖的水解和运输相关，参与植物对生物及非生物胁迫的应答。我们在前期研究中发现：低温和脱落酸能诱导番茄细胞壁蔗糖转化酶抑制蛋白（INVINH1）的表达；与野生型相比，INVINH1 RNA干扰的转基因番茄表现出对低温胁迫更强的耐受性。为揭示INVINH1在调节植物耐受低温胁迫中的功能，本项目拟开展以下研究：分析番茄INVINH1在营养器官中的表达模式；低温处理对基因表达、酶活性和糖含量的影响；观察INVINH1过量表达及RNA干扰的转基因植株在低温处理后的表型，检测与低温耐受相关的生理指标；鉴定调节INVINH1基因表达的转录因子；探讨INVINH1在调节植物耐寒性中的机理。本研究将有助于阐明蔗糖转化酶抑制蛋白在调节植株抵抗低温胁迫中的功能，从而为遗传改良农作物的耐寒能力提供理论依据。

Invertase, related with sucrose hydrolysis and transport, play diverse roles in responses to biotic and abiotic stresses. In our preliminary study, the expression of cell wall invertase inhibitor (INVINH1)in tomato was induced by cold and abscisic acid treatment. Interestingly, compared with the wile type, the transgenic tomato silencing the expression of INVINH1 showed an enhanced tolerance to cold stress. To indicate the role of INVINH1 in plant cold tolerance, the proposal will be carried out in tomato as follows: find out the expression pattern of INVINH1 in the vegetative organs of tomato; compare the change of gene expression pattern, invertase activity and sugar content between in wild type and in transgenic tomato before or after cold treatment; observe the phenotype of the transgenic tomato overexpressing and silencing INVINH1 after cold treatment, and detect the physiological index related with plant cold resistance; search the transcription factors which regulate the expression of INVINH1; investigate the possible mechanism by which INVINH1 regulates the plant tolerance after cold treatment. This study will uncover the funtion of cell wall invertase and the effect of sugar content on plant tolerance to cold stress, which provides further scientific basis for genetic improvement of crops'cold tolerance.