自然条件下铁皮石斛的正常生长发育依赖于植物根系与菌根真菌建立的共生体。铁皮石斛自然资源已近枯竭，目前依靠人工栽培的方法生产药材，栽培中未使用菌根真菌。前期研究表明菌根真菌MF23能缩短铁皮石斛种植周期，大幅度提高药材产量，具有良好的应用前景。对菌根栽培生产药材的质量和药效缺乏了解，制约了菌根技术在药用植物栽培生产中的推广应用。针对这个问题，本项目计划研究MF23菌根栽培与非菌根栽培生产的药材在质量和药效方面的差别，通过比较揭示MF23对药材质量和药效的影响。研究内容包括：一、研究菌根真菌对药材中多糖、联苄和小分子代谢产物的影响；二、研究菌根真菌对药材抗氧化和抗肿瘤免疫调节作用的影响。通过研究明确菌根栽培铁皮石斛的质量特征及药理作用，为药材的安全使用提供保证，有利于铁皮石斛资源的开发和利用；同时能为铁皮石斛菌根栽培技术的推广应用奠定理论基础。

Dendrobium officinale (Orchidaceae) is an endangered medicinal plant as well as the sole origin of Dendrobii Officinalis Caulis (DOC). Scientific research has confirmed that D. officinale can’t ensure the normal growth and development without forming mycorrhiza with fungi. Currently, DOC production relies on D. officinale artificial cultivation, in which mycorrhizal fungi are not used. Our early research has shown that mycorrhizal fungi MF23 can shorten plant growth time and greatly improve the output. Therefore, application of MF23 to D. officinale cultivation has bright prospect. However, MF23 has not been widely used for lack of understanding that whether MF23 affects the quality and efficacy of DOC. The purpose of this project is to solve the question. Stems of mycorrhizal (MDOC) and non-mycorrhizal (DOC) cultivation D. officinale, are used in the research including: 1/ determination of polysaccharide, naringenin and bibenzyls, and fingerprint analysis of small molecule chemical composition; 2/ pharmacological research of ethyl acetate and water extracts on antioxidation and anti-tumor immune regulation. The results of these studies will prove the pharmacological actions of MDOS and reveal its quality characteristics, which can ensure the safety use of MDOS. At the same time, these results will be good for the application and population of mycorrhizal cultivation technique for D. officinale, and for other endangered plants.