母源基因在减数分裂中发挥重要作用，而纺锤体组装相关的母源基因对卵和胚胎的染色体倍性维持及胚胎发育潜能尤为关键。申请者前期对人类着床前早期胚胎，以及原始生殖细胞的转录组进行系统分析，研究发现BOD1、LIMA1在卵和早期胚胎高表达，而在早期雌性原始生殖细胞（减数分裂前）和八细胞后（合子基因激活）的阶段表达量均较低，提示其在卵成熟及早期胚胎发育过程发挥重要作用。有文献报道这两个基因对有丝分裂过程纺锤体组装和染色体排列具有重要的作用，但在卵母细胞减数分裂及胚胎发育过程中的作用未见相关报道。本项目拟利用小鼠卵母细胞体外敲低BOD1、LIMA1表达，观察卵成熟、胚胎发育及整倍体性，用捐赠人未成熟卵进行功能验证，并探讨其在反复卵成熟障碍、早期胚胎发育阻滞中作用。该研究将有助于理解卵及早期胚胎发育机制，为生殖医学临床疑难病例诊疗提供新思路和手段。

Maternal genes play an important role in meiosis, spindle assembly associated maternal genes are particularly critical for the euploid maintenance and embryonic development. Applicant's previous studies systematically characterized the transcriptome of human primordial germ cells (PGCs) and pre-implantation embryos at single-cell and single-base resolutions. Two genes (BOD1, LIMA1) were found in a similar expression pattern with maternal genes, which are highly expressed in eggs and early embryos, but low in early PGC (pre-meiotic) and after 8-cell (post-ZGA) stages, suggesting that they may play a crucial role in egg maturation and early embryo development process. It has been reported that these two genes are crucial for spindle assembly and chromosome arrangement, but their role in meiosis and embryonic development remains unknown. This project intends to knock down the expression of BOD1 and LIMA1 in mouse eggs and observe the maturation and euploidy of the eggs, embryo development potential. With donated human immature eggs, the functions of these two genes are to be verified. Furthermore，the cause of repeated eggs maturation arrest and early embryo developmental arrest is planned to evaluated in IVF clinic. The study will help understand the mechanisms of egg and early embryo development and provide new ideas for clinical diagnosis and treatment.