蒙药清肝9味散通过MAPKs通路调控MMPs/TIMPs体系抗肝纤维化的作用及机制研究

肝纤维化是慢性肝炎发展至肝硬化的必经病理过程,肝硬化在我国发病率居高不下，且病死率高，是危害人类健康的常见疾病之一，因此其治疗和发病机制成为了肝病领域的研究热点。研究表明，肝纤维化在一定程度上可以逆转,从而使"慢性肝炎-肝纤维化-肝硬化-肝癌"这一进展过程得以终止。肝星状细胞(HSC)的活化与增殖是肝纤维化发生的关键环节,对MMPs/TIMPs体系的调节起着重要作用。目前与HSC活化有关的信号转导机制是普遍的研究焦点。我们通过长期的临床工作及前期研究中发现，蒙药清肝九味散对肝纤维化患者有着确切的治疗作用。此次研究应用该药物治疗肝纤维化大鼠模型，检测肝纤维化相关血清学指标，同时观察药物干预的大鼠肝脏组织和细胞培养液中MMPs/TIMPs体系的变化及其对MAPKs信号通路相关蛋白的影响，并对MAPKs通路相关蛋白进行筛选、验证。期望通过该研究能明确蒙药清肝九味散治疗肝纤维化的作用机制

Liver fibrosis is a key prossess in the development from chronic hepatitis to cirrhosis of the liver pathological process, There are a lot of hepatitic cirrhosis patients in our country, and the case fatality rate is high, it is one of the common disease which endangering human health, so its pathogenesis and treatment has become a hotspot in the field of liver disease research.Studies have shown that hepatitic fibrosis can be reversed in some extent, so that the "chronic hepatitis - hepatitic fibrosis - hepatitic cirrhosis - liver cancer," the progress of the process may be terminated.The activation of hepatic stellate cells (HSC) is a key link in the process of liver fibrosis, and proliferation of hepatic stellate cells plays an important role in the regulation of MMPs / TIMPs.The signal transduction associated with HSC activation mechanism is a common research focus.After our long-term research and clinical work ,Qing Gan Jiu Wei San has a definite therapeutic effect on liver fibrosis patients. In this study, we used the drug to treat rat model of hepatic fibrosis and detect the serum markers related to hepatic fibrosis. Meanwhile, we observed the changes of MMPs / TIMPs in liver tissue and cell culture medium of drug intervention and its correlation with MAPKs signal pathway Protein and the MAPKs related proteins were screened and verified. It is expected that this study will clarify the mechanism of Mongolian medicine Qing Gan Jiu Wei San in the treatment of liver fibrosis, and provide scientific basis for the treatment of liver fibrosis.