本项目针对实现流域水生态管理亟需解决水生生物快速检测的实际需求，以河流着生藻类为研究对象，结合流域水生态功能分区方案，采用传统形态学鉴定和DNA 条形码方法，研究不同条形码候选序列（cox I、rbcL、rbcSp、UPA、28S-rDNA 和ITS）在着生藻类分类中的鉴别能力，筛选并确立核心DNA条形码，构建辽河流域着生藻类实物图像库和与其对应的DNA条形码库；在此基础上，结合环境DNA metabarcoding方法和高通量测序技术，建立辽河着生藻类快速、标准化鉴定体系，比较分析基于DNA方法和传统分类方法的生物多样性结果，探明群落结构特征、生物多样性与水环境因子之间的关系，以物种分布和数量变化趋势为基准，开展着生藻类生物完整性（P-IBI）评价，探讨该技术在河流生态系统健康（REH）研究中的有效性，为流域水生生物分类、多样性保护及水生态环境管理提供科学依据。

As the realization of water ecological management necessitates us to meet the actual needs of rapid detection of aquatic organisms in rivers，the project uses periphytic algae as research object, combining basin water ecological function regionalization program, to study the ability to identify algae of single and combinations of the six gene fragments( cox I, rbcL, rbcSp, UPA, 28S-rDNA and ITS). We will screen and determine the core universal primers of DNA barcoding of periphytic algae in Liaohe River Basin, combining traditional morphological identification with molecular biology methods, to build image database and the corresponding DNA barcode library of periphytic algae in Liao River Basin. On this basis, using metagenomics approaches, rapid identification and biodiversity analysis of periphytic algae in Liaohe River will be conducted, to clarify the relationship between genetic diversity and species diversity of periphytic algae. Using variation trend of distribution and abundance of species as a benchmark, we will compare and analyse the relationship between genetic diversity, species diversity and water environmental factors, and carry out biological integrity assessment (P-IBI) of periphytic algae, to evaluate the effectiveness of the technique in the river ecosystem health (REH) research. The aim of the project is to provide fundamental data for aquatic organism taxonomy, biodiversity conservation and aquatic ecological environment management.