运输刺激作为畜牧生产中常见的应激原，可导致畜产品质量降低，动物死亡率增加，已成为制约我国肉牛养殖业快速发展的关键因素之一，其发生机制的研究受到国内外科研工作者的广泛关注。目前，在人和小鼠等模式生物中，miRNA已经作为一种有效标靶，用于相关疾病的早期诊断和预后研究，但在家畜尤其是涉及肉牛运输应激诊断及发生机制的研究国内未见报道。因此，本研究以肉牛为研究对象，采用高通量的全基因组芯片、qRT-PCR、Northern blot等现代分子生物学技术，拟构建运输应激前后肉牛血清miRNA差异表达谱，结合生物信息学技术，分析运输应激相关的差异表达miRNAs及其靶基因，应用基因沉默和过表达等技术，对目标miRNA和靶基因进行鉴定和功能验证，以期获得运输应激miRNA诊断标志物，并揭示目标miRNA在调控肉牛运输应激反应的作用，为明晰运输应激发生的分子机制奠定理论基础。

The transport stimulation is a common stressor in livestock production, and it prevent the rapid development of beef breeding industry which is due to the transport stimulation could reduce the quality of animal products and increase animal mortality. Scientist pay attention to the mechanism research on transport stress. Among human, mouse and other model organisms, miRNA has been regarded as an valid target tag on the early diagnosis and prognosis in the related diseases, while there still have no such study around the early diagnosis and mechanism on beef transport stress. In this study, beeves are as the objects, and to construct a differential expression profiling of serum miRNAs on transport stressed beeves compared with normal beeves by high-throughput microarray, qRT-PCR and Northern blot technology. Then to analyse differentially expressed miRNAs and its target genes which both are related to transport stress. Silencing and over-expression methods will be using to verify the functional and regulational mechanism of the target differentially expressed miRNAs and its target genes. By analyzing the above experimental results could obtain the miRNA diagnosis maker on transport stress and reveal the roles of target miRNAs when the beeves are on the state of transport stress. The study results could play a key role on clarifying the molecular mechanisms on transport stress.