肉鸡股骨头坏死关节软骨细胞凋亡的内质网应激调控机理的研究

股骨头坏死（femoral head necrosis，FHN）是快速生长肉鸡最常见的腿病之一，不仅导致经济损失，而且危害动物福利。股骨头关节软骨细胞凋亡是该病的重要病理特征之一。关节软骨细胞的活力和表型维持直接影响着肉鸡FHN的病理进程。内质网应激（endoplasmic reticulum stress，ERS）在关节软骨细胞凋亡过程中发挥重要的调控作用，但其调控机理至今仍不详。本研究在建立肉鸡FHN模型和原代关节软骨细胞培养的基础上，应用蛋白质组学和生物信息学等技术分析ERS对关节软骨细胞基因表达的影响，ERS调控信号分子对关节软骨细胞表型维持与凋亡的作用途径和反馈作用机制，结合骨参数、骨代谢标志物检测，组织病理学观察，探讨肉鸡FHN发生中ERS导致关节软骨细胞凋亡，引发关节软骨损伤的机理，阐明ERS在肉鸡FHN发生中的作用和临床意义，为肉鸡FHN发病机理和防控的研究提供理论依据。

Femoral head necrosis (FHN) is one of the most common leg disorders in fast-growing broiler chickens, and leads to economic loss and poultry welfare compromise. Apoptosis of chondrocytes in the articular cartilage of femoral head is one of the most important characteristic pathological changes in FHN broilers. Viability and phenotype maintenance of articular chondrocytes directly affect FHN pathogenesis in broilers. Endoplasmic reticulum stress (ERS) play key regulatory roles in the process of articular chondrocyte apoptosis, but its regulatory roles and molecular mechanisms are still unclear. In the present study, FHN experimental model is built, articular chondrocytes are cultured, the effect of ERS on the expression of articular chondrocyte genes is assessed, the pathyways and feedback mechanisms of phenotype maintance and apoptosis of articular chondrocytes regulated by ERS are determined by proteomic and bioinformatics analyses, the changes of histopathology and bone metabolic markers and parameters are evaluated. The purpose of the present study is to investigate the mechanisms that ERS results in articular chondrocyte apoptosis and articular cartilage damage, to clarify the roles and clinical significance of ERS in the development of FHN and provide the theoretical evidence for pathogenesis, control and treatment of FHN in broilers.