丝氨酸蛋白酶抑制剂（serine protease inhibitors，SPI）广泛存在于哺乳动物、植物和微生物体内。多种痘病毒编码SPI，其中正痘病毒属编码的SPI包括SPI-1、SPI-2/CrmA、SPI-3。SPI-1具有决定宿主范围的作用，SPI-2具有抑制宿主炎症反应和细胞凋亡等功能。进行鸡痘病毒（fowlpox virus，FPV）282E4全基因组测序及序列分析发现其基因组中存在5个SERPIN基因，本研究拟根据痘病毒科现有研究基础，建立稳定表达SERPIN蛋白的DF1细胞系，通过形态学观察、MTT检测细胞活性、Caspase活性检测、流式细胞术等筛选出具有抑制宿主细胞凋亡活性的SERPIN，进一步构建缺失SERPIN的重组FPV接种DF1、DF1/SERPIN细胞进行验证，免疫共沉淀鉴定与SERPIN相互作用的蛋白，为禽痘病毒SERPIN蛋白生物学功能研究提供实验数据。

Serine protease inhibitors (SPI) is exists widely in mammals, plants and microorganisms. It has been reported that a variety of poxviruses encodes SPI gene, including SPI-1, SPI-2 / CrmA and SPI-3. SPI-1 has a role in the decision host range, SPI-2 could inhibits apoptosis and inflammatory response of host and so on. By sequencing the whole genome of the fowlpox virus (FPV) 282E4, and then we found five SERPIN genes. The project was based on the research results of poxviridae to establish a DF1 cell lines that could stable expression the SERPIN protein. Then we screening the SERPIN that inhibit host apoptotic activity by morphological observation, cell activity detected with MTT, Caspase-3、8、 activity assay and flow cytometry. We further build the recombinant FPV of missing SERPIN, to verification apoptotic activity by inoculated DF1, DF1/SERPIN cell with FPV△SERPIN. Further we utilizing Co-immunoprecipitation experiments identified protein that interactions with SERPIN. In summary, this result can provide experimental data for the biology function of the SERPIN protein of avian pox virus.