自噬与癫痫发作后神经元损伤密切相关。在前期研究证实以益肾填精法为主的茸菖胶囊抗痫增智疗效肯定、保护神经元优势突出的基础上，提出"茸菖胶囊可通过调控神经元自噬发挥抗癫痫及神经保护作用"假说。本研究以海马神经元自噬为切入点，以惊厥刺激、自噬及其活性调控通路激活、神经元损害为主线，采用中药脑脊液药理学技术，以原代培养的胎鼠海马神经元无镁诱导的反复惊厥样放电为模型，设正常组、模型组、中药脑脊液组和3-MA对照组。在无镁损害后1.5、3、12、24、72h及第6天检测：①神经元损伤及其自噬体的形态学改变；②自噬相关基因与蛋白（Beclin1、vps34、Bcl-2、Atg5、LC3、Cathepsin B）的表达；③自噬调控信号通路相关基因与蛋白（p110α、p- AMPK、Akt/mTOR、p-Akt/mTOR）表达。阐明益肾填精法抗痫和神经保护作用的自噬调控机制，为临床应用提供实验依据。

Autophagy is closely correlated with neuronal injury of epilepsy.Based on the earlier research confirmed that Rongchang capsule with the enriching kidney essence method has exact antiepileptic effect and protection neurons, the hypothesis that Rongchang capsule play antiepileptic effect and neural protection role by regulating neurons autophagy was put forward. This study is intended to take autophagosome as key point;mainly based on convulsions stimuli neuronal injury,autophagy,signaling pathway and activity regulation;using cerebrospinal fluid pharmacology of Chinese materiamedica method,primary cultured neurons from hippocampus of neonate rats with recurrent epileptiform discharges induced by magnesium-free-treatment.Divided into normal group, model group,TCM cerebrospinal fluid group (Rongchang capsule) and 3-Methyladenine(3-MA) control group. At different time (1.5h, 3h, 6h, 12h, 24h, 72h and 14d) after Mg2+-free treatment observing:(1) morphology changes of damage neurons and autophagy body change; (2) the changes of autophagy-associated genes and proteins (Beclin1, vps34 Bcl-2, and Atg5, LC3，Cathepsin B) expression; (3) the changes of autophagy signal regulation pathways-related genes and proteins (p110 alpha, p-AMPK, Akt/mTOR, p-Akt/mTOR) expression. Expound the autophagy regulation mechanism of the enriching kidney essence method with antiepileptic and neuroprotective effect so as to provide experiment evidence for clinic.