在分子水平上对单细胞进行实时、原位、动态检测方法对于探寻细胞生命活动过程中生物分子的性质和功能具有重要的意义。本项目提出一种基于多芯光纤光操控技术和宽带FT-CARS光谱技术实现的新型的非标记单细胞显微成像方法。使用多芯光纤光操控系统以非接触、无损伤的方式对液态环境中特定的单细胞的三维空间位置精准操控和姿态调整，实现细胞内部激发光束的快速扫描。通过宽带FT-CARS光谱技术获取细胞内不同位置生物分子在400-1500cm-1范围内的完整CARS指纹光谱，提供化学选择性和成像对比度，获取单细胞内生物分子空间分布图像。为获取活细胞的精细结构，研究单细胞内生物分子的功能，进而在分子水平上揭示细胞活动的基本规律提供一种高性能、多功能的新型研究平台，具有重要的应用价值和广泛的应用前景。

For exploring the properties and functions of biomolecules in the processes of cellular living activities, the real-time, in situ and dynamic evaluation method of single cells at the molecular level has great significance. In this project, one novel unlabeled single-cell microscopy is proposed, which is based on the optical manipulation with multi-core optical fiber and broadband Fourier transform coherent anti-Stokes Raman scattering (FT-CARS) spectroscopy. In liquid environment, the three-dimensional position and posture of specific single cell are accurately manipulated and adjusted by the optical manipulation system with multi-core fibers without contact and damage. The rapid scanning of the excitation beam is achieved in three-dimensional space inside the cell. In different positions of a cell, the complete CARS fingerprint spectrums of intracellular biomolecules, in the range of 400-1500 cm-1, are simultaneously acquired with a broadband FT-CARS spectroscopy, which provides the chemical selectivity and imaging contrast. The spatial distribution images of biomolecules in a single cell are obtained. The proposed non-label microscopy is one novel versatile research platform with high performance for acquiring the fine structures of single living cell, studying the functions of biomolecules of single cell, and further revealing the basic laws of cell activities at the molecular level, which has important application value and broad prospects.