慢性鼻-鼻窦炎（CRS）是鼻腔鼻窦黏液高分泌性疾病，但其机制未知。已证实，慢性下呼吸道炎性疾病是由于嗜中性粒细胞（NEU）弹性蛋白酶（HNE）致肿瘤坏死因子-α转化酶（TACE）活化，促进TGF-α分泌增多，激活下游分子EGFR，从而导致黏蛋白表达增高。我们前期研究发现CRS鼻窦黏膜上皮中HNE、TGF-α及EGFR较正常组织表达增高。结合我国CRS患者以NEU浸润为主，提示HNE-TACE- TGF-α-EGFR信号通路与CRS黏蛋白过度表达密切相关。本研究拟进一步检测该通路在CRS组织中的表达情况，通过建立鼻黏膜原代细胞和大鼠鼻腔黏液高分泌模型，检测该信号通路与黏蛋白表达的关系。该研究的进行，旨在探讨HNE在CRS中黏蛋白过度表达的作用，同时也为CRS的免疫治疗提供重要的新方向、新靶点。

Chronic rhinosinusitis(CRS) is a disease with high mucus of nasal navity and sinus,however,its mechanism is unknown.It has been confermed that human neutrophils elastase can activate TNF-αconverting enzyme(TACE),then TGF-α is increased by TACE stimulation and EGFR is activated at last,which result in the increase of mucin.In our previous research,the expression of HNE、TGF-α、EGFR were higher in CRS patients than normal ones.Chineses patients with CRS are characterized by NEU inflammation, implying that there is close relative between HNE-TACE-TGF-α-EGFR signal pathway and mucin secretion in CRS.In our study,we will detect the expression of the signal pathway in CRS,then investigate the relation of both with primary nasal epithelium cells and high mucus rats models. The aim of our study is to illuminate the role and mechinism of HNE to increase mucus secretion in CRS and supply the new perspective to cure CRS patients.