研究调控植物耐热相关基因转录表达的分子机制具有重大理论和实践意义。在前期工作中，在拟南芥和水稻中都证明了多个光信号元件双B-box（DBB）型锌指蛋白家族成员的表达受到热胁迫的诱导，并发现其中一个成员AtBBX18能降低拟南芥的耐热性，且能调控二乳糖甘油酯合成酶DGD1和热击转录因子HsfA2等耐热相关基因的转录表达。然而水稻中受热胁迫诱导表达的OsBBX在耐热信号途径中的生物学功能以及调控耐热相关基因转录表达的分子机理所知甚少，因此，拟开展以下几方面研究：1）系统研究OsDBB基因响应热胁迫的组织细胞表达特征；2）阐明热诱导表达的OsDBB蛋白调控水稻响应热胁迫的生物学功能；3）试图从分子水平上阐明热胁迫诱导表达的OsDBB调控DGD和Hsfs转录表达的分子机制，对探明水稻耐受高温胁迫的分子遗传机制具有重要的科学意义。

It's important theoretical and practical significance to elucidate the regulatory molecular mechanisms involved in gene expression related to thermotolerance. In previous work, we found the transcriptional expression of several members of double B-box zinc finger protein subfamily were induced by heat stress in Arabidopsis and rice. Besides, AtBBX18, as a member of this subfamily, is a negative regulator of thermotolerance by regulated the transcriptional expression of digalactosyldiacylglycerol synthase 1 (DGD1), and heat shock factor HsfA2 in Arabidopsis. However, those heat stress induced OsDBBs are unknown determinants involved in heat stress responses in rice, and how OsDBB involve in thermotolerance molecular signalling is poorly understood. Therefore, this research intends to carry out the following research areas: (1) systematically analysis of the cellar and organic expression of heat stress-induced OsDBB in response to heat stress. (2) systematically study thermotolerance phenotypes of heat stress-induced OsDBB transgenic rice plants; (3) we attempt to clarify the molecular mechanism of OsDBB in the regulation of DGD and Hsfs expression in rice. Above experiments will have important scientific significance to deeply understand the molecular genetic mechanism in response to hight temperature in rice.