南方水稻黑条矮缩病毒（SRBSDV）由白背飞虱以持久增殖型方式进行传播。该病毒在介体昆虫内能够诱导形成病毒复制工厂—病毒原质，主要由病毒编码的P5、P6、P9-1蛋白及其介体因子参与形成。其中，P6蛋白在病毒侵染过程中较早表达，且分别和P5、P9-1蛋白进行特异性互作，在病毒原质形成过程中发挥重要作用。本项目拟以SRBSDV P6蛋白为主要诱饵蛋白，通过酵母双杂交钓取白背飞虱中肠内与病毒复制相关的介体因子。利用介体靶标蛋白的基因克隆，亚细胞定位和RNA干扰等技术弄清介体因子调控SRBSDV在白背飞虱中肠形成病毒原质的分子机制，为明确SRBSDV在昆虫体内的复制机理奠定基础。

Southern rice black-streaked dwarf virus (SRBSDV) is transmitted by white-backed planthopper (WBPH; Sogatella furcifera Horváth) in a persistent-propagative manner. During SRBSDV infection in its insect vector, viroplasm, putative sites of viral replication, contained the nonstructural viral protein P5, P6, P9-1 and insect vector proteins. The P6 is innitially expressed, and interacts with P5 and P9-1 in insect vector, respectively. All these results demonstrated that P6 was essential for viroplasm formation and viral replication. In this study, yeast two hybrid experiments will be used to identify insect proteins interacting with SRBSDV P6 in the midgut. Gene clone, subcellular localization and RNA interference will be used to know about how insect proteins regulate SRBSDV form viroplasm in the midgut of insect vector. Results of this study will be useful in revealing the replication mechanisms of SRBSDV in its insect vector, which will facilitate the development of effective control measures against Southern rice black-streaked dwarf disease.