microRNA-21介导的雪旺细胞自噬在周围神经修复中的作用及机制研究

本项目以神经损伤后雪旺细胞发生自噬为切入点，在课题组前期对小分子RNA（microRNA）在神经修复过程中的作用机制的研究基础上，观察microRNA-21对雪旺细胞自噬的调节作用。应用荧光共聚焦技术、透射电镜、蛋白定量、神经示踪等技术及神经功能评估方法，探究BCL-2作为microRNA-21的靶基因，通过BCL-2 /Beclin 1-PI3K途径影响雪旺细胞自噬的分子机制，并在植入转染microRNA-21雪旺细胞的受损神经进行大鼠神经功能评估，以及检测损伤灶雪旺细胞增殖情况、神经再生相关信号分子活性、轴突生长等情况在修复过程中的改变。本项目旨在探讨在神经损伤过程中雪旺细胞内转录后用调控参与雪旺细胞自噬 的作用机制，丰富神经损伤再生理论，为治疗神经损伤提供新的治疗思路与理论依据。

This project by schwann cells after nerve injury occurred autophagy as the breakthrough point, based on the research of small rnas (mirna) in the process of nerve repair mechanism research, observe the adjustment of mirnas - 21 on schwann cell autophagy. Application of confocal fluorescence technology and transmission electron microscopy (sem), protein quantitative and neural tracer technology and neural function evaluation methods, explore the affection of the BCL - 2 on schwann cells as micrornas - 21 target genes, the molecular mechanisms of autophagy,via BCL-2 /Beclin 1-PI3K. And implante transfection micrornas - 21 schwann cells damaged nerve of the rat to neural function assessment, and detect injuries focal schwann cell proliferation, signal molecule activity related to nerve regeneration, growth of axons. This project Aims to explore the process of nerve injury moderate schwann cell after transcription regulation in cells involved in the mechanism of action of schwann cell autophagy, nerve injury regeneration theory, offers a new therapy for treatment of nerve damage train of thought and theory basis.