基于ERK及P38/MAPK信号通路探讨清热除湿法对HPV阳性宫颈癌细胞调节蛋白作用机制研究.

宫颈癌是女性第二大恶性肿瘤，持续性HR-HPV感染引发CIN会进一步发展为宫颈癌，是唯一病因明确，可以预防的癌症。治疗HPV感染对于防治宫颈癌至关重要。目前尚无疗效确切的治疗药物，国外主张预防性疫苗的应用，但由于应用周期长、远期疗效和副作用尚未不明确，限制了其应用。基于中医治未病的理念，我院名老中医郭志强教授运用“清热除湿法”创立紫柏凝胶外用方治疗HPV感染，疗效显著，但作用机制尚不明确。本项目拟采用分子生物学技术，旨在研究：①HPV（+）宫颈癌细胞中ERK及P38/MAPK 信号通路与癌症调节蛋白E6、E7、P53、Rb之间的关系。②“清热除湿法”是否通过此通路影响癌症调节蛋白。③外用方中有效基团的确立，及各成分对该通路和调节蛋白作用是否一致。通过以上研究为中药治疗HPV感染、预防宫颈癌提供机制研究依据。

Cervical cancer is the second malignant tumor in women , persistent HR-HPV infection caused CIN ,and the further the cervical cancer.Cervical cancer is the only one cancer that has the clear cause and can be prevented. The national old TCM professor GuoZhiqiang founded the "topical prescription of heat addition mechanism of wet “ which can treat the HR-HPV infection and related diseases, it has been used for more than 30 years ,it has confirmed the obvious curative effect, but the mechanism is not clear. The project intends to use molecular biology technology, the research aims to study the heat addition mechanism of wet compound single drug in the extraction effect of effective components in HPV positive cervical cancer cells through inhibition of the MAPK/ERK signaling pathway, decreased expression of E6 oncogene protein and the pro apoptotic factor P53 protein expression, decreased cell proliferation and apoptosis ability. To establish" Chinese medicine play a major therapeutic effect of Zibai gel in the external side, whether there is a synergistic effect and inhibitory effect of traditional Chinese medicine. It provides an important basis for clinical treatment and prevention of HPV infection and cervical disease.