杆状病毒表面展示系统(BDS)可通过将目标抗原展示在病毒粒子囊膜表面用于疫苗开发，但传统BDS存在展示效率不高，展示目标因子过少，细胞培养昂贵等缺点而无法满足现代疫苗发展需要。本项目拟以课题组开发的家蚕杆状病毒多基因表达系统为基础，构建出一套高效经济的新型家蚕杆状病毒多基因展示系统(SBMDS)。该系统能将多达10个目标基因展示于病毒囊膜外表面，可展示多个抗原，也可同时展示免疫增强因子、免疫逃避因子、甚至蛋白免疫佐剂，以显著提高免疫效率，从而为发展高效多价动物疫苗提供平台。如以猪流感病毒(SIV)为模型，通过构建同时展示SIV的血球凝集素(HA)、唾液酸苷酶(NA)及哺乳动物细胞侵袭素和免疫逃避因子等多种蛋白的展示病毒，并确定其免疫原性，为今后发展出有效防治SIV疫苗奠定基础。本研究充分发挥多基因展示的效能优势和家蚕饲养的成本优势，在新型疫苗研发平台构建和预防动物传染等方面具有重要意义。

Baculovirus display system (BDS) can be used to develop vaccine through displaying target antigens on the envelope surface of baculovirus. However, traditional BDS has many disadvantages, such as low efficiency of display, few in number of displayed antigens, and high cost of insect cells culture, which greatly impede the application of BDS. This project plans to construct a new efficient, inexpensive silkworm-baculovirus multigene display system (SBMDS) on the base of silkworm-baculovirus multigene expression system (SBMES) formed by our group. The designed system is able to efficiently display 10 target antigens on the outer surface of virus envelope, supplying a nice technique platform for the production of animal polyvaccines. SBMDS can not only display multi antigens, but also display immunologic enhance factors, immunologic escape factors, and even immunologic adjuvants to provide higher immune efficiency. Here, we choose swine influenza virus (SIV) as an experimental model, to construct a multigene chimeric display virus containing the hemagglutinin (HA) and neuraminidase (NA) of SIV-H1N1, the NA of SIV-H1N2, the HA of SIV-H3N2, the invasion and the decay-accelerating factor (DAF) of mammalian cell, and detect its immunogenicity. This research has great advantages on the high efficiency of multigene display and the low cost of silkworm feed. It will provide strong base for the building of polyvaccine platform, as well as the preventing of animal zymosis.