动物性比率调节是进化生物学一个至关重要的研究问题。Hamilton的性比模型虽然极大地解释了很多动物性比偏雌的现象，但是越来越多的实验发现Hamilton性比模型的局限性，尤其是在聚果榕传粉小蜂中发现小蜂雄性后代数量不随着进蜂量增加而改变的现象，挑战了Hamilton的性比模型。基于传粉榕小蜂先产雄性卵事实，本文提出从榕小蜂控制储精囊开闭来调控性比的机制。本研究假设榕小蜂在产卵过程中先产未受精卵（雄性），在某一时刻储精囊打开，排出精子，小蜂开始产受精卵（雌性）。本实验将通过基因组与转录组测序分析，找出调控聚果榕传粉榕小蜂储精囊开启的基因，并通过解剖比较不同产卵阶段榕小蜂储精囊的开启情况，和相关基因表达差异来检验榕小蜂通过储精囊开闭调节性比率变化这一假说。

The sex ratio adjustment mechanism is a key note question of Evolution. Although, Hamilton’s Extraordinary Sex Ratio model can partially explain the female bias sex ratio, more and more field experiment about fig pollinating wasps challenged the Hamilton’s sex ratio model. Ceratosolen fusciceps lay the same number of male offspring no matter how many foundress in the same figs. Given that fig pollinating wasps lay male eggs first, we propose the on-off sex ratio mechanism of fig pollinating wasps. We suppose that during the whole ovulating process, female wasps lay the unfertilized eggs (male) at first and at some point, spermatheca release the sperm and then wasps begin to lay fertilized eggs (female). In this project, we are going to analyze the genome and transcriptome data of the wasps and find the genes that regulate the spermatheca, and compare the anatomical differences and gene expression differences among different ovulation periods to verify the hypothesis: Sex ratio of fig pollinating wasp is controlled by the manipulation of spermatheca.