α-SNAP及其介导的膜泡运输在大豆与大豆孢囊线虫互作中的机制研究

大豆孢囊线虫病是影响大豆生产的最具毁灭性的病害之一，利用抗病品种是最有效的绿色防控措施之一。大豆抗孢囊线虫关键位点Rhg1在抗病育种中的应用最广泛。前期的研究表明，Rhg1拷贝数目增加导致该位点内三个基因表达量的升高，从而提高大豆抗病性。作为膜泡运输的重要成员，α-SNAP是Rhg1位点中的关键基因，但其抗病分子机制还不是很清楚。本项目将在前期工作的基础上深入研究α-SNAP抗病的分子机制。初步的实验结果表明，α-SNAP与SAK5和SAK6两个蛋白互作，α-SNAP基因的表达量在侵染位点受到调控。我们将进一步研究α-SNAP与SAK5和SAK6的互作机制，解析α-SNAP基因受诱导表达的分子机制，揭示α-SNAP介导的膜泡运输在取食细胞形成中的功能。项目旨在阐明α-SNAP的抗病机制和调控网络，扩展我们对植物与寄生线虫互作机制的认识，为作物抗病育种提供重要的理论依据。

Soybean cyst nematode (SCN, Heterodera glycines) is one of the most damaging pathogen of soybean. The use of SCN-resistant cultivars is the most effective means of managing SCN population levels in the field. Rhg1 is widely used in SCN breeding program. Previous studies revealed that copy number variation that elevates the expression of three genes at Rhg1 confers SCN resistance. As a critical component of vesicle trafficking, α-SNAP (alpha soluble NSF [N-ethylmaleimide-sensitive factor] attachment protein) plays an important role in Rhg1-mediated SCN resistance. But little is known about the mechanism by which these α-SNAPs contribute to resitance. In this project, we’ll dissect the molecular basis of α-SNAPs in SCN resistance. Our studies revealed that α-SNAP interacted with SAP5 and SAP6, and the expression of α-SNAP is upregulated in nematode-infected tissue. We’re going to identify the functions of α-SNAPs through the analysis of α-SNAP-interactive proteins. The upsteam components that regulate the expression of α-SNAPs will be identified. And the role of vesicle trafficking in the feeding cell formation will also be investigated. Overall, our studies will help us understand the molecular mechanism underlying the functions of α-SNAP during plant-nematode interaction, providing us a potential means to engineer crop resistance to the nematode.