苜蓿对重要害虫-牛角花齿蓟马（Odontothrips loti)的耐害机制研究

牛角花齿蓟马（Odontothrips loti）是我国北方苜蓿的主要害虫，严重影响苜蓿的产量与质量，已成为苜蓿生产的重要威胁和障碍。通过苜蓿抗蓟马育种是防治该虫最有效的途径之一，而明确的抗性机制是高效抗虫育种的基础。本项目将以此虫为研究对象，以筛选的抗、感蓟马苜蓿无性系为材料，研究不同虫口压力对苜蓿的抗性表现的影响，解决苜蓿抗蓟马研究中最适接虫量问题；从蓟马对苜蓿生长、光合、营养分配的影响以及叶片超显微结构的变化着手，研究分析苜蓿对蓟马的耐害机制，并从理论上阐明苜蓿抗蓟马的耐害机制及其密切相关的重要特性；在此基础上建立苜蓿抗蓟马的筛选鉴定技术，为苜蓿抗蓟马鉴定和抗蓟马分子育种创造捷径，为苜蓿抗蓟马研究提供基础理论，也为我国苜蓿生产中蓟马危害的可持续治理提供理论依据。

Odontothrips loti is an important pest insect infesting alfalfa in northern China that reduces alfalfa yield and quality seriously, and has now become the major problem for alfalfa production. Application of resistant alfalfa variety has been demonstrated as one of the most effective ways to prevent and to control O.loti, whereas to make the resistance mechanisms clear is the basis of breeding resistant alfalfa efficiently. The resistant and susceptible alfalfa clones to thrips and O.loti will used as test material to study the effect of different densities on the resistant capability of alfalfa to ensure the optimum quantity for the study of alfalfa's resistance to thrips.The research will began with the effect of O.loti on the resistant and susceptible alfalfa in growth, photosynthesis, nutrition distributing in tissues and the ultra-structural of leaf. Based on these studies, discovering the tolerant mechanism of alfalfa and its closely related characteristics, and a screening and identification method will be established to make a shortcut for resistance-thrips molecular breeding, and to provide theoretical basis for the breeding of anti-thrips alfalfa and the realization of sustainable O. loti management in alfalfa production.