我国土壤和蔬菜邻苯二甲酸酯（PAEs）污染普遍，严重危害农产品安全和人体健康。我们前期筛选获得了PAEs低累积菜心品种和降解PAEs植物内生菌，明确了接种该菌能进一步显著降低菜心体内PAEs含量，但其机理尚不清楚。本项目拟研究内生菌对根际土壤微界面PAEs环境行为的作用机制；探讨内生菌对菜心吸收累积PAEs的生理生化的影响过程；通过考察内生菌定殖的菜心体内PAEs亚细胞分配、代谢产物分布、代谢酶活性，查明内生菌促进菜心体内代谢PAEs的酶学机制；采用转录组学和蛋白组学比较分析内生菌定殖的菜心在代谢PAEs过程中的关键基因（或蛋白）差异表达，阐明内生菌促进菜心代谢PAEs的分子机制。从而将揭示植物内生菌降低菜心吸收累积PAEs的机理，为高效利用污染土壤生产安全农产品提供科学依据。

Substantial phthalate esters (PAEs) pollution in soils and vegetables has been found in many areas across China, which has adversely affected the safety of agricultural products and thus increases health risk for humans through dietary ingestion. Therefore, there is an urgency to reduce uptake and accumulation of PAEs by vegetables. In our previous study, we have screened out low-PAE accumulating cultivar of Chinese flowering cabbage (Brassica parachinensis L.) and PAEs-degrading endophytic bacterium, and ensured that the inoculation of Chinese flowering cabbage with this strain can significantly reduce the concentration of PAEs. However, the mechanisms involved are not yet well understood. The present project will study the mechanisms of action of endophytic inoculation on environmental behavior of PAEs at the micro-interface in rhizospheric soil. Moreover, this project will further investigate the effects of inoculation of endophyte on the physiological and biochemical processes of Chinese flowering cabbage involved in lowering accumulation of PAEs, elucidate the enzymatic mechanisms of enhanced biotransformation of PAEs in endophyte-inoculated Chinese flowering cabbage by investigating the subcellular distribution of PAEs and related metabolic products and the activities of PAE-degrading enzymes, reveal the molecular biology mechanisms of enhanced transformation of PAEs in Chinese flowering cabbage through comparative transcriptome and iTRAQ proteome analysis for identifying differentially expressed genes and proteins of endophyte-inoculated Chinese flowering cabbage. It will provide an important insight in endophyte-aided reduction of uptake and accumulation of PAEs in Chinese flowering cabbage and scientific basis for the safe qualities of agricultural products.