CCP1在高尔基体形态维持和花粉管生长中的功能研究

COG（conserved oligomeric Golgi）复合体是COPI囊泡拴系因子。我们前期的研究表明COG亚基COG3/8通过有效栓系COPI囊泡来调控高尔基体的形态结构和运输，并影响花粉管的生长，但其分子调控机制还不清楚。目前，该项目的前期数据表明COG8与钙调素结合蛋白CCP1（COG-interacting Calmodulin-binding Protein1）互作，ccp1-2和cog3/8突变花粉呈现相似的高尔基体形态缺陷，同时，CCP1与钙调素Calmodulin2/4/6也直接互作。该项目计划通过分子与生化，细胞，遗传及药物处理等方法，解析CCP1在COPI囊泡栓系/融合、高尔基体形态维持和运输以及花粉管生长中的作用。这将有助于提高人们对植物高尔基体，尤其是可能参与COPI囊泡栓系/融合的Ca2+-Calmodulin信号通路的认知。

COG complex (conserved oligomeric Golgi) is a tethering factor for COPI vesicles. Our recently work demonstrated that COG subunits COG3/8 modulated Golgi morphology, Golgi transport, and pollen tube growth through appropriate tethering of COPI vesicles, but the molecular mechanism remains elusive. So far, acquired data of this project showed that COG8 interacts with CCP1 (COG-interacting Calmodulin-binding Protein 1), and Golgi morphology displayed similar defects in ccp1-2 and cog3/8 mutant pollen. At the same time, CCP1 directly interact with Calmodulin2/4/6 as well. Using a combination of molecular and biochemical, cell biology, genetics, and pharmaceutical approaches, our specific objectives are to characterize CCP1 in COPI tethering/fusion, Golgi morphology maintenance, Golgi trafficking, and pollen tube growth. In doing so, we hope to improve our understanding of plant Golgi apparatus, especially the possible involvement of Ca2+-Calmodulin signaling pathway in COPI tethering/fusion.