水稻是重要的粮食作物，其胚乳中含有大量的储藏蛋白，是人类摄取蛋白质的主要来源。储藏蛋白的含量、储藏部位及其形式对稻米的营养品质和加工品质有直接影响，因此储藏蛋白的研究是稻米品质改良的一个重要研究方向。水稻谷蛋白是主要的储藏蛋白，以前体形式合成和转运，最终在储藏液泡中剪切成为酸性和碱性亚基，其转运途径受多个基因的共同调控，任何基因的突变都导致其不能正确剪切，从而出现前体累积的表型，因此谷蛋白前体累积突变体是研究水稻储藏蛋白胞内转运调控机制的理想材料。本研究以谷蛋白前体累积突变体glupa-1为材料，通过图位克隆定位目标基因，鉴定调控储藏蛋白转运的新基因，通过多种生物学方法研究其功能，解析其作用机理，揭示其参与的调控途径。该项目有利于阐明蛋白胞内转运调控机制，具有重要的理论意义，同时对于提高水稻营养价值、改良稻米品质具有极其重要的应用价值。

Rice is an important food crop, it contains a large amount of storage protein in endosperm, which is the main source of protein intake by humans. The storage protein content, storage location and its form have a direct impact on the nutritional quality and processing quality of rice. Therefore, the study of storage protein is an important research of rice quality improvement. The glutelin is the main storage protein, which is synthesized and transported in the form of precursor, and finally cleavage into acidic and basic subunits in the storage vacuole. The transport pathway of glutelin is regulated by multiple genes, and the mutation of any gene causes it accumulated as the precursor. Therefore, the glutelin precursor accumulation mutant is an ideal material for studying the regulation mechanism of intracellular transport of rice storage proteins. In this study, the glutelin precursor mutant glupa-1 was used as a material to map the target gene by map cloning, identify new genes regulating the transport of storage proteins, study its function through various biological methods, analyze its mechanism and regulatory pathways. This study is helpful for elucidating the regulation mechanism of proteins transport, which has important theoretical significance and great application value for improving the nutritional value of rice and improving rice quality.