中蔗新品系根系抗旱相关的加权基因共表达网络分析和核心基因发掘及抗旱分子机理研究

干旱是制约我国甘蔗生产的重要因素，利用品种的抗旱性是解决干旱问题的经济、安全的最有效措施。本申请项目以亚热带农业生物资源保护与利用国家重点实验室培育的中蔗新品系强耐旱的中蔗9号和不耐旱的中蔗2号为研究材料，通过研究甘蔗抗旱根系表型性状、生理生化指标，以及转录组测序，利用WGCNA建立与甘蔗根系抗旱表型及生理相关联的基因共表达网络，鉴定与根系抗旱密切相关的基因共表达模块，挖掘模块中与目标性状关联度高的核心基因，并弄清其参与干旱胁迫的调控通路，在此基础上初步阐述甘蔗根系抗旱的表型、生理和分子机理。本研究将促进对旱胁迫下甘蔗特定生物学过程的分子机制及其调控途径的理解，以期为利用甘蔗抗旱种质资源改良甘蔗的抗旱性和为抗旱新品种的培育提供理论依据。

Drought has become an important factor restricting sugarcane production in China. The utilization of resistant cultivars is the most economical, safe, and effective method to solve the problem of drought. In this project, the two new cultivars （drought-resistant ZZ 9 and non-drought-resistant ZZ 2，coming from the same parents）cultivated by State Key Laboratory for Conservation and Utilization of Subtropical Agricultural Biological Resources，will be selected as the research materials. By studying the phenotypic characters, physiology and biochemistry, and transcriptome sequencing of sugarcane drought resistant roots, we will construct a co-expression network of weighted genes associated with the drought resistant phenotype and physiology of sugarcane roots by WGCNA, and identify genes co-expression modules closely related to root drought resistance. In addition, some hub genes involved in drought stress regulation pathways will be excavated from the modules with the highest correlation with target traits，and the regulation pathway of drought stress will be clarified. On this basis, the phenotypic, physiological and molecular mechanism of drought resistance in sugarcane root system will be preliminarily described. This study will promote the understanding of the molecular mechanism and regulatory pathway of specific biological process of Sugarcane under drought stress, in order to improve the drought resistance of sugarcane by using the sugarcane drought resistant germplasm resources，and provide theoretical basis for the cultivation of new drought resistant varieties.