应用 DiI 对人类胚胎三叉神经中枢核团研究

三叉神经的感觉纤维将头面部的一般体性感觉（触觉，压觉，温度觉，痛觉）情报传导到中枢神经系。关于人类胚胎头面部的三叉神经支配领域以及三叉神经节细胞在神经节内的局在性分布已有报道。但这种局在性分布能否保持在三叉神经的上行投射路中的各级核团，以及这些核团的形态、细胞分布，脑内相关性结构的关系如何，这些问题待我们解决。三叉神经痛是周围神经痛最常见的一种，患病率为182/10万，发病率是4.7/10万人，目前三叉神经半月节的选择性定位损伤是治疗三叉神经痛的重要手段之一，因此三叉神的中枢投射、定位对该外科手术具有一定的理论和实践意义。课题内容包括：①建立人类胚胎三叉神经运动核的正常位置及细胞分布模型；②DiI追踪实验来定位三叉神经感觉核的形态及细胞分布；③明确三叉神经运动核及感觉核的细胞发育；④各类核团的三维立体图形构建。

The sensory fibers of Trigeminal transmitted the general feeling information (touch, pressure sensation, temperature sensation, pain) from head and face to the central nervous system .the distribution of the trigeminal field of innervation and the trigeminal ganglion cells in the ganglion have been reported on the face and head in human fetus However, this relationship between the distribution of whether the road to maintain the upward projection of the trigeminal nerve nuclei at all levels, as well as the morphology of these nuclei, cell distribution, the brain of the correlation structure, has not sloved. Trigeminal neuralgia is the most common form of peripheral neuropathic pain, the prevalence was 182/100 000, the incidence rate was 4.7/10 million, semilunar ganglion selective positioning of the trigeminal nerve injury is an important means of treatment of trigeminal neuralgiaone, so the trigeminal God in the central projection, the positioning of the surgery has certain theoretical and practical significance. The topics to be covered include: ① To establish the human embryonic trigeminal nerve motor nuclei in the normal position and cell distribution model; ②applying DiI tracing experiments to locate the trigeminal sensory nuclear morphology and cell distribution; ③development of the motor nuclei and sensory nucleus in the trigeminal nerve cell; ④ To reconstruction The various types of nuclei of three-dimensional graphics。