acrAB-tolC基因在大肠埃希菌对消毒剂苯扎溴铵和四环素类抗菌药物交叉耐药机制中的作用研究

研究消毒剂和四环素类抗菌药物之间的交叉耐药机制，为耐药性控制提供科学依据。选择消毒剂苯扎溴铵和四环素类抗菌药物为研究对象, 在前期研究表明二者交叉耐药且与外排泵机制高度相关的基础上，拟深入研究主要外排泵耐药基因acrAB-tolC在这一交叉耐药性中的作用。将大肠埃希菌质控株ATCC25922苯扎溴铵和土霉素诱导耐药株与野生耐药株的外排泵相关基因测序，分析研究野生耐药株及诱导前后大肠埃希菌qacA、qacC、qacE、emrE、mdfA、theA、acrA、tetB等基因变异，acrAB-tolC外排泵基因的克隆与表达，从而确认acrAB-tolC在该交叉耐药机制中的作用，为养殖业和医学临床正确使用消毒剂和抗菌药物提供理论依据，减少"超级细菌"的产生，保障养殖业的健康发展、食品安全和维护人类健康。

The aim of this study is to explore the mechanism of the cross resistance between Benzalkonium bromide and Tetracyclines, so as to provide scientific evidences of their resistance. As the cross resistance of Disinfectant and Tetracyclines, which is closely associated with some efflux pumps, has been proved by our studies, the function of acrAB-tolC in the cross resistance will be deeply studied in this research. To sequence the efflux pump genes of the Escherichia coli quality control strains ATCC25922 which is benzalkonium bromide and oxytetracycline-induced resistance strains and wild resistant strains, analysis and research the genovariation of wild resistant strains and qacA、qacC、qacE、emrE、mdfA、theA、acrA、tetB gene of Escherichia coli before and after the induction, then analysis and research the change of Efflux pump AcrAB-TolC, to confirm the acrAB-tolC’s function in this cross resistance mechanisms. So, this research will provide theory support for correctly using disinfectant and antibacterial agents so as to avoid the development of "superbugs", ensure the healthy promotion of breeding industry, food safety and keep human healthy.