基于IDO1介导Kyn/AhR通路调控Th17/Treg细胞分化探讨消癌解毒方抑制结直肠癌的作用机制

近年来我国结直肠癌的发病率、死亡率呈明显上升趋势。消癌解毒方是国医大师周仲瑛教授多年临床实践形成的经验方，临床研究证实具有抑制结直肠癌的作用，但机制尚不清楚。最新研究表明，免疫逃逸是肿瘤发生发展的关键环节，IDO1是免疫逃逸形成的关键因子，Th17/Treg细胞分化失衡是免疫逃逸的重要机制，IDO1介导Kyn/AhR通路可以抑制Th17细胞、激活Treg细胞。前期研究发现，消癌解毒方可以抑制Treg细胞，改善T细胞免疫功能。据此，我们提出该方可能是通过干预IDO1介导Kyn/AhR通路，调控Th17/Treg细胞分化，发挥抑制结直肠癌的作用。本研究以癌毒病机理论为指导，运用分子生物学、系统生物学、药理学等技术方法，通过体内和体外实验，探讨IDO1介导Kyn/AhR通路调控Th17/Treg细胞分化参与免疫逃逸的机制，阐明消癌解毒方抑制结直肠癌的作用机制，为中医药防治结直肠癌提供科学依据。

In recent years, the incidence rate and mortality rate of colorectal cancer in China have been increasing obviously. The“XiaoAi JieDu Recipe” is prescribed by TCM master professor Zhou Zhongying based on his clinical experience. It has been proved clinically to inhibit colorectal cancer, but its mechanism remains to be explored. Studies show that immune escape is a key link in the occurrence and development of malignant tumors, IDO1 is a key factor of immune escape,the imbalance of Th17/Treg cell differentiation is an important mechanism of tumor immune escape,IDO1 can inhibit Th17 cells and activate Treg cells through Kyn/AhR pathway. It was found that XiaoAi JieDu recipe could inhibit Treg cells and improve T cell immune function. Therefore, we suggest that this recipe may play a role in inhibiting colorectal cancer by intervening IDO1 mediated Kyn/AhR pathway to regulate Th17/Treg cell differentiation.Under the guidance of the theory of cancer-toxin pathogenesis, this study is about to explore the mechanism of IDO1 mediated Kyn/AhR pathway regulating Th17/Treg cell differentiation in vivo and in vitro by molecular biology, system biology, pharmacology and other technical methods, to clarify the mechanism of XiaoAi JieDu recipe in inhibiting colorectal cancer.The research will provide scientific basis for the prevention and treatment of colorectal cancer by traditional Chinese medicine.