基于AGEs-RAGE信号转导通路探讨鬼箭羽治疗糖尿病肾病的作用机制

临床研究表明鬼箭羽治疗糖尿病肾病（Diabetic kidney disease, DKD）具有较好的疗效，但对于其作用机制的研究却鲜有报道。随着网络药理学与生物信息学的发展，使得对中药作用机制的研究有了关联度较高的切入点。本研究在已有工作基础上，提出“鬼箭羽治疗DKD是经由AGEs-RAGE信号转导通路实现”的科学假说，并拟通过体内、体外实验在不同作用模式下进行干预研究，包括：（1）在体动物水平验证：建立DKD的大鼠模型，结合组织水平HE染色、免疫组化等技术获取鬼箭羽通过AGEs-RAGE信号通路，以及下游的促炎因子与/或血管内皮生长因子来调控治疗DKD的在体水平证据；（2）体外细胞水平验证：通过DKD模型原代培养肾脏肾小球系膜细胞和足细胞，检测相关靶基因的含量，进一步观察给药以及相应的激动剂与阻断剂后所产生的影响，从分子层面阐明鬼箭羽治疗DKD潜在的作用机制。

Guijianyu has achieved good clinical effects in the treatment of diabetic kidney disease (DKD), but the research on its related mechanisms is scarce. With the development of network pharmacology and bioinformatics, the research on the mechanism of Chinese medicine has a highly relevant entry point. Based on the existing work, this research puts forward the scientific hypothesis that "DKD treatment by ghost arrow feathers is achieved through the AGEs-RAGE signal transduction pathway", and plans to conduct intervention studies under different modes of action through in vivo and in vitro experiments, including: (1) Verification at the in vivo animal level: establish a rat model of diabetic nephropathy, combine with tissue-level HE staining, immunohistochemistry and other techniques to explore whether Guijianyu treats diabetic nephropathy through the AGEs-RAGE signaling pathway, as well as downstream pro-inflammatory factors and / Or vascular endothelial growth factor to regulate and establish research evidence at the in vivo level; (2) In vitro cell level verification: primary culture of renal glomerular mesangial cells and podocytes through diabetic nephropathy model to detect the content of related target genes , To further observe the effects of administration and the corresponding agonists and blockers, and to clarify the potential mechanism of the effect of ghost feathers on the molecular level.