阐明鱼类高不饱和脂肪酸（HUFA）合成调控机制是解决配合饲料中植物油替代鱼油问题的基础。目前，有关HUFA合成关键酶基因的转录调控和转录后调控机制研究已取得较好进展，但关于调控HUFA合成的细胞信号通路尚未见报道。在哺乳类的研究显示，G蛋白偶联受体120（GPR120）细胞信号通路与脂肪合成调控有关，但其是否参与HUFA合成调控尚不清楚。我们利用黄斑蓝子鱼肝细胞系（SCHL）进行初步研究发现，其中可能存在一条与HUFA合成调控有关的GPR120细胞信号通路：GPR120-AMPK-SREBP-1-HUFA合成酶（∆6/∆5 Fad和Elovl5）通路。本项目拟以SCHL为对象，借助细胞学和分子生物学技术，系统探究GPR120细胞信号通路在HUFA合成调控中的作用及机制。研究成果将为揭示鱼类HUFA合成调控机制增添新内容，将是GPR120细胞信号通路参与脊椎动物HUFA合成调控的首次报道。

Clarification for the regulatory mechanism of teleost HUFA biosynthesis is the fundamental step to relieve the crisis of the fish oil (FO) replacement with vegetable oil (VO) in compound feed. At present, the regulation on teleost HUFA biosynthesis has obtained excellent achievements at transcriptional and post-transcriptional level, while in cellular signal transduction level, there was little research on such field. Recently, there is a report in mammal that the G-protein-coupled receptor (GPR120) cellular transduction pathway is involved in adipogenesis, whether this signal pathway regulates HUFA biosynthesis is unknown at present. The primary experiment on Siganus canaliculatus hepatocyte line (SCHL) suggested that there could be one GPR120 signaling pathway, GPR120-AMPK-SREBP-1-∆6/∆5 Fad and Elovl5, which might be involved in the regulation of HUFA biosynthesis. Thus, present study is performed systematically on SCHL cell line with the technology of cell culture and molecular biology to identify the regulatory mechanism of such signal pathway to HUFA biosynthesis. Finally, the research achievement would provide novel content for regulatory mechanism in teleost HUFA biosynthesis, which could be the first report of GPR120 signaling pathway involved in regulation on vertebrate HUFA biosynthesis.