鬼臼毒素是一种成功商品化的天然木脂素，在治疗尖锐湿疣、抗癌与抗肿瘤方面具有显著的疗效，在临床已得到广泛应用。项目在已证明濒危藏药桃儿七低温适应以及低温促进鬼臼毒素积累的基础上，拟利用双向聚丙烯酰胺凝胶电泳(2D-PAGE)、质谱(MS)、高效液相色谱(HPLC)和实施定量PCR(qRT-PCR)等技术，研究桃儿七低温适应过程中差异表达蛋白质的数量、生化特性以及生物学功能，并根据差异蛋白质表达量和功能分析结果，分析差异蛋白质的表达量与关键酶活性、关键基因表达量的相互关系，从“蛋白质-基因-调控网络”三个方面探明桃儿七低温适应以及低温促进鬼臼毒素积累的分子机制。研究结果将为更好地认识和理解鬼臼毒素的代谢与调控过程、提高组织细胞培养过程中鬼臼毒素产率、指导桃儿七规模化种植、保护野生桃儿七资源等方面提供理论基础与技术支撑。

Podophyllotoxin (PPT) is a successful commercialization lignans, it and its derivatives are widely used in the clinical setting such as genital warts, cancer and anti-tumor, etc. In our previous study, we have proved that endangered Tibetan medicine Podophyllum hexandrum Royle (P. hexandrum) could adapt the low temperature, and the PPT content could be enhanced under cold stress. With the aim of investigating into the molecular mechanisms, the number and biochemical properties and biological functions of differentially expressed proteins in P. hexandrum will be detected by two-dimensional polyacrylamide gel electrophoresis(2D-PAGE), mass spectrometry (MS) and bioinformatics analysis; And PPT content, key enzymes activity and genes expression will be detected by high performance liquid chromatography (HPLC) and quantitative Real-time PCR (qRT-PCR); Then the relationship between differentially expressed proteins and key enzymes and genes will be analyzed. Ultimately, the molecular mechanisms of P. hexandrum cold-adaption and cold enhancing the PPT accumulation would be revealed from three aspects "protein -gene-regulatory network". The results would provide more information and theoretical foundation on understanding the metabolic and regulatory processes of PPT, improving PDT production in ell tissue culture, guiding large-scale cultivation and protecting the wild resources of P. hexandrum.